Explore
Validate
LearnH00002273-M01
antibody from Novus Biologicals
Targeting: FHL1
bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00002273-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002273-M01, RRID:AB_538823
- Product name
- Mouse Monoclonal FHL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FHL1 - four and a half LIM domains 1
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Four-and-a-half LIM domain proteins inhibit transactivation by hypoxia-inducible factor 1.
Carbonic anhydrase III and four-and-a-half LIM protein 1 are preferentially oxidized with muscle unloading.
Hubbi ME, Gilkes DM, Baek JH, Semenza GL
The Journal of biological chemistry 2012 Feb 24;287(9):6139-49
The Journal of biological chemistry 2012 Feb 24;287(9):6139-49
Carbonic anhydrase III and four-and-a-half LIM protein 1 are preferentially oxidized with muscle unloading.
Chen CN, Ferrington DA, Thompson LV
Journal of applied physiology (Bethesda, Md. : 1985) 2008 Nov;105(5):1554-61
Journal of applied physiology (Bethesda, Md. : 1985) 2008 Nov;105(5):1554-61
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - Analysis of FHL1 expression in transfected 293T cell line by FHL1 monoclonal antibody (M01), clone 2A9.Lane 1: FHL1 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FHL1 Antibody (2A9) [H00002273-M01] - FHL1 monoclonal antibody (M01), clone 2A9. Analysis of FHL1 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: FHL1 Antibody (2A9) [H00002273-M01] - Detection limit for recombinant GST tagged FHL1 is approximately 0.3ng/ml as a capture antibody.
