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LearnPA5-27141
antibody from Invitrogen Antibodies
Targeting: FHL1
bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
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- Product number
- PA5-27141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FHL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, mouse muscle, mouse lung. Predicted reactivity: Pig (100%), Sheep (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FHL1 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FHL1 polyclonal antibody (Product # PA5-27141) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FHL1 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FHL1 Polyclonal Antibody (Product # PA5-27141) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FHL1 Polyclonal Antibody (Product # PA5-27141). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 Polyclonal Antibody (Product # PA5-27141) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
