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LearnPA5-32161
antibody from Invitrogen Antibodies
Targeting: FHL1
bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- PA5-32161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FHL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat. Predicted reactivity: Mouse (93%), Rat (94%), Pig (97%), Sheep (95%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FHL1 using 30 µg of Jurkat lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a FHL1 polyclonal antibody (Product # PA5-32161) at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FHL1 Polyclonal Antibody detects FHL1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. FHL1 Polyclonal Antibody (Product # PA5-32161) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FHL1 Polyclonal Antibody detects FHL1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. FHL1 Polyclonal Antibody (Product # PA5-32161) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FHL1 Polyclonal Antibody detects FHL1 protein at cytosol and nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. FHL1 Polyclonal Antibody (Product # PA5-32161) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using FHL1 (Product # PA5-32161) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
