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LearnNB100-1461
antibody from Novus Biologicals
Targeting: FHL1
bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- NB100-1461 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1461, RRID:AB_10003395
- Product name
- Goat Polyclonal FHL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress.
Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
Zhang L, Chen X, Sharma P, Moon M, Sheftel AD, Dawood F, Nghiem MP, Wu J, Li RK, Gramolini AO, Sorensen PH, Penninger JM, Brumell JH, Liu PP
Nature communications 2014 Mar 11;5:3430
Nature communications 2014 Mar 11;5:3430
Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA
The Journal of biological chemistry 1999 Sep 17;274(38):27083-91
The Journal of biological chemistry 1999 Sep 17;274(38):27083-91
Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA
The Journal of biological chemistry 1999 Sep 17;274(38):27083-91
The Journal of biological chemistry 1999 Sep 17;274(38):27083-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FHL1 Antibody [NB100-1461] - (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FHL1 Antibody [NB100-1461] - Proteins interacting with HACE1 accumulate in Hace1-/-sTAC heart. Representative western blots show increased accumulation of some of the HACE1-interacting proteins in the Hace1-/-sTAC myocardium. n=3 mice per group, error bars represent s.e.m., P-value between KO and WT sTAC as indicated (one-way analysis of variance). GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms4430), licensed under a CC-BY licence.
