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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NBP2-12814 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PI4KB/PI4KIII beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.
Su Y, Wang P, Shen H, Sun Z, Xu C, Li G, Tong T, Chen J
Journal of cell science 2018 Mar 16;131(6)
Journal of cell science 2018 Mar 16;131(6)
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: PI4KB/PI4KIII beta Antibody [NBP2-12814] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PI4KB antibody NBP2-12814 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PI4KB was also immunoprecipitated by rabbit anti-PI4KB antibody NBP2-12815, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
