15342-1-AP

antibody from Invitrogen Antibodies

Targeting: MRPL9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15342-1-AP

Provider

Invitrogen Antibodies

Product name

MRPL9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAPVVTAP GRALLRAGAG RLLRGGVQEL LRPRHEGNAP DLACNFSLSQ NRGTVIVERW WKVPLAGEGR KPRLHRRHRV YKLVEDTKHR PKENLELILT QSVENVGVRG DLVSVKKSLG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKIQTKA GEATVKFLKS CRLEVGMKNN VKWELNPEIV ARHFFKNLGV VVAPHTLKLP EEPITRWGEY WCEVTVNGLD TVRVPMSVVN FEKPKTKRYK YWLAQQAAKA MAPTSPQI (1-267 aa encoded by BC004517)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 15342-1-AP (MRPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 15342-1-AP (MRPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed MCF-7 cells using 15342-1-AP (MRPL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed MCF-7 cells using 15342-1-AP (MRPL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded tissue slide using 15342-1-AP (MRPL9 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded tissue slide using 15342-1-AP (MRPL9 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 15342-1-AP (MRPL9 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 15342-1-AP (MRPL9 antibody) at dilution of 1:100 (under 40x lens).