IC7821A-100
antibody from Novus Biologicals
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC7821A-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-22 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL-22 and recombinant rat IL-22 and no cross-reactivity with recombinant human IL-10.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-22 in Human PBMCs stimulated to induce Th17 Cells by Flow Cytometry. CD4+ Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342), 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL), 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL), and 10 ng/mL Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) for 5 to 7 days, then re-stimulated with 50 ng/mL PMA and 200 ng/mL Calcium ionomycin for 2 to 4 hours or (B) resting CD4+ PBMCs were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human IL-22 APC-conjugated Monoclonal Antibody (Catalog # IC7821A). Quadrant markers were set based on isotype control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.