366704
antibody from BioLegend
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 366704 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#366704, RRID:AB_2565568
- Product name
- PE anti-human IL-22
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant protein expressed in E. Coli
- Description
- IL-22 is a cytokine that is structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF, also known as the IL-10-related T cell-derived inducible factor. IL-22 belongs to a family of cytokines with limited homology specifically to IL-10, IL-19, IL-20, IL-24, IL-26, the IFN-λs, IL-28A, IL-28B, and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Its activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor family. Two types of IL-22 binding receptors have been discovered: a membrane-bound receptor and a soluble receptor that are encoded by different genes. IL-22 is produced by Th17 cells and Th22 cells. The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 2G12A41
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- PMA/ionomycin stimulated (six hours in the presence of monensin) human peripheral blood mononuclear cells were stained with CD4 APC, fixed, permeabilized, and stained with IL-22 (clone 2G12A41) PE (top image) or mouse IgG2a, ?» PE (bottom image).
- Conjugate
- Yellow dye