MAB582
antibody from R&D Systems
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB582 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-22 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JJY9
- Isotype
- IgG
- Antibody clone number
- 140301
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dermal γδ T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity.
IL-22 defines a novel immune pathway of antifungal resistance.
Interleukin 22 (IL-22) plays a protective role in T cell-mediated murine hepatitis: IL-22 is a survival factor for hepatocytes via STAT3 activation.
Jiang X, Park CO, Geddes Sweeney J, Yoo MJ, Gaide O, Kupper TS
PloS one 2017;12(1):e0169397
PloS one 2017;12(1):e0169397
IL-22 defines a novel immune pathway of antifungal resistance.
De Luca A, Zelante T, D'Angelo C, Zagarella S, Fallarino F, Spreca A, Iannitti RG, Bonifazi P, Renauld JC, Bistoni F, Puccetti P, Romani L
Mucosal immunology 2010 Jul;3(4):361-73
Mucosal immunology 2010 Jul;3(4):361-73
Interleukin 22 (IL-22) plays a protective role in T cell-mediated murine hepatitis: IL-22 is a survival factor for hepatocytes via STAT3 activation.
Radaeva S, Sun R, Pan HN, Hong F, Gao B
Hepatology (Baltimore, Md.) 2004 May;39(5):1332-42
Hepatology (Baltimore, Md.) 2004 May;39(5):1332-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-22 in Mouse Th17 Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 μg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/mL), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/mL) for 5 days were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (Catalog # MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012). View our protocol for Staining Intracellular Molecules.