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LearnIC7821A-100
antibody from R&D Systems
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC7821A-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-22 APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL-22 and recombinant rat IL-22 and no cross-reactivity with recombinant human IL-10.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
Q9GZX6- Isotype
- IgG
- Antibody clone number
- 142928
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Mycobacterium tuberculosis-Specific IL-21+IFN-γ+CD4+ T Cells Are Regulated by IL-12.
Trained immunity in newborn infants of HBV-infected mothers.
CX₃CL1 (fractalkine) and its receptor CX₃CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin.
Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation.
IL-22 suppresses IFN-γ-mediated lung inflammation in asthmatic patients.
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y, Wu C
PloS one 2016;11(1):e0147356
PloS one 2016;11(1):e0147356
Trained immunity in newborn infants of HBV-infected mothers.
Hong M, Sandalova E, Low D, Gehring AJ, Fieni S, Amadei B, Urbani S, Chong YS, Guccione E, Bertoletti A
Nature communications 2015 Mar 25;6:6588
Nature communications 2015 Mar 25;6:6588
CX₃CL1 (fractalkine) and its receptor CX₃CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin.
Staumont-Sallé D, Fleury S, Lazzari A, Molendi-Coste O, Hornez N, Lavogiez C, Kanda A, Wartelle J, Fries A, Pennino D, Mionnet C, Prawitt J, Bouchaert E, Delaporte E, Glaichenhaus N, Staels B, Julia V, Dombrowicz D
The Journal of experimental medicine 2014 Jun 2;211(6):1185-96
The Journal of experimental medicine 2014 Jun 2;211(6):1185-96
Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation.
Schuetz A, Deleage C, Sereti I, Rerknimitr R, Phanuphak N, Phuang-Ngern Y, Estes JD, Sandler NG, Sukhumvittaya S, Marovich M, Jongrakthaitae S, Akapirat S, Fletscher JL, Kroon E, Dewar R, Trichavaroj R, Chomchey N, Douek DC, O Connell RJ, Ngauy V, Robb ML, Phanuphak P, Michael NL, Excler JL, Kim JH, de Souza MS, Ananworanich J, RV254/SEARCH 010 and RV304/SEARCH 013 Study Groups.
PLoS pathogens 2014 Dec;10(12):e1004543
PLoS pathogens 2014 Dec;10(12):e1004543
IL-22 suppresses IFN-γ-mediated lung inflammation in asthmatic patients.
Pennino D, Bhavsar PK, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham SR, Cavani A, Eyerich K, Chung KF, Schmidt-Weber CB, Eyerich S
The Journal of allergy and clinical immunology 2013 Feb;131(2):562-70
The Journal of allergy and clinical immunology 2013 Feb;131(2):562-70
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
Chan YR, Chen K, Duncan SR, Lathrop KL, Latoche JD, Logar AJ, Pociask DA, Wahlberg BJ, Ray P, Ray A, Pilewski JM, Kolls JK
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5
IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
Tang XZ, Jo J, Tan AT, Sandalova E, Chia A, Tan KC, Lee KH, Gehring AJ, De Libero G, Bertoletti A
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3142-52
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3142-52
Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
Schmidt J, Iversen AK, Tenzer S, Gostick E, Price DA, Lohmann V, Distler U, Bowness P, Schild H, Blum HE, Klenerman P, Neumann-Haefelin C, Thimme R
PLoS pathogens 2012;8(11):e1003042
PLoS pathogens 2012;8(11):e1003042
ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY
Scandinavian journal of immunology 2011 Apr;73(4):330-7
Scandinavian journal of immunology 2011 Apr;73(4):330-7
Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C
Immunology 2011 Apr;132(4):540-8
Immunology 2011 Apr;132(4):540-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-22 in Human PBMCs stimulated to induce Th17 Cells by Flow Cytometry. CD4+ Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342), 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL), 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL), and 10 ng/mL Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) for 5 to 7 days, then re-stimulated with 50 ng/mL PMA and 200 ng/mL Calcium ionomycin for 2 to 4 hours or (B) resting CD4+ PBMCs were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human IL-22 APC-conjugated Monoclonal Antibody (Catalog # IC7821A). Quadrant markers were set based on isotype control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
