16-7222-82

antibody from Invitrogen Antibodies
Targeting: IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Provider product page for 16-7222-82
Blocking/Neutralizing
Other assay

Antibody data

Product number
16-7222-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
IL-22 Monoclonal Antibody (IL22JOP), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells, NK cells, and other T cells. Compared to IL-6 or TGF beta, IL-23 can induce greater levels of IL-22 in in vitro-differentiated Th17 cells. This observation suggests that IL-22 may be secreted by more fully differentiated Th17 cells in vivo. Recently, it was demonstrated that IL-22 could protect hosts from bacterial infection of the lungs and gut. Moreover, it has been reported that anti-CD3/CD28-induced production of IL-22 by PBMCs was elevated significantly in asthma patients compared to control patients. Flow cytometric analysis also showed that the frequencies of IL-17+IL-22+ CD4 T cells were increased in PBMCs from patients with ankylosing spondylitis and rheumatoid arthritis. IL22JOP is published to recognize rhesus IL-22. Applications Reported: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22. Applications Tested: The ND50 of IL22JOP, as determined by the inhibition of IL-10 induction in COLO205 cells by IL-22, is 10-40 ng/mL, in the presence of 0.2 ng/mL recombinant human or mouse IL-22. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
IL22JOP
Vial size
100 µg
Concentration
1 mg/mL
Storage
4° C
IL22 protein structure - 16-7222-82 shown in red.