50-7229-41

antibody from Invitrogen Antibodies

Targeting: IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

50-7229-41

Provider

Invitrogen Antibodies

Product name

Anti-IL-22 Monoclonal Antibody (22URTI), eFluor 660, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22. Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th17-polarized human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

22URTI

Vial size

25 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Chen X, Wang Y, Wang J, Wen J, Jia X, Wang X, Zhang H
Oncology letters 2018 Jul;16(1):253-261

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Downregulation of RUNX3 moderates the frequency of Th17 and Th22 cells in patients with psoriasis.

Fu D, Song X, Hu H, Sun M, Li Z, Tian Z
Molecular medicine reports 2016 Jun;13(6):4606-12

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Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.

Wang WB, Yen ML, Liu KJ, Hsu PJ, Lin MH, Chen PM, Sudhir PR, Chen CH, Chen CH, Sytwu HK, Yen BL
Stem cell reports 2015 Sep 8;5(3):392-404

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T helper cell subsets specific for Pseudomonas aeruginosa in healthy individuals and patients with cystic fibrosis.

Bayes HK, Bicknell S, MacGregor G, Evans TJ
PloS one 2014;9(2):e90263

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Elevated frequencies of circulating Th22 cell in addition to Th17 cell and Th17/Th1 cell in patients with acute coronary syndrome.

Zhang L, Wang T, Wang XQ, Du RZ, Zhang KN, Liu XG, Ma DX, Yu S, Su GH, Li ZH, Guan YQ, Du NL
PloS one 2013;8(12):e71466

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Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.

Zhang L, Li YG, Li YH, Qi L, Liu XG, Yuan CZ, Hu NW, Ma DX, Li ZF, Yang Q, Li W, Li JM
PloS one 2012;7(4):e31000

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Induction and effector functions of T(H)17 cells.

Bettelli E, Korn T, Oukka M, Kuchroo VK
Nature 2008 Jun 19;453(7198):1051-7

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23 Recombinant Protein (Product # 14-8239-63) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or Cell Stimulation Cocktail plus Protein Transport Inhibitors (Product # 00-4975-03) (right) for 6 hours. Cells were intracellularly stained with Anti-Human CD4 PE (Product # 12-0047-42) and Anti-Human IL-22 eFluor® 660 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Viable cells, as dtermined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.