Explore
Validate
Learn500-P211BT-1MG
antibody from Invitrogen Antibodies
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P211BT-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-22 Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-22. Anti-Human IL-22-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-22 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human 4-1BBL (500-P211) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-22. Western Blot: To detect Human IL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible development reagents the detection limit for Recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P211BT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 2 x 500 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.
Bouhaddou M, Memon D, Meyer B, White KM, Rezelj VV, Correa Marrero M, Polacco BJ, Melnyk JE, Ulferts S, Kaake RM, Batra J, Richards AL, Stevenson E, Gordon DE, Rojc A, Obernier K, Fabius JM, Soucheray M, Miorin L, Moreno E, Koh C, Tran QD, Hardy A, Robinot R, Vallet T, Nilsson-Payant BE, Hernandez-Armenta C, Dunham A, Weigang S, Knerr J, Modak M, Quintero D, Zhou Y, Dugourd A, Valdeolivas A, Patil T, Li Q, Hüttenhain R, Cakir M, Muralidharan M, Kim M, Jang G, Tutuncuoglu B, Hiatt J, Guo JZ, Xu J, Bouhaddou S, Mathy CJP, Gaulton A, Manners EJ, Félix E, Shi Y, Goff M, Lim JK, McBride T, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, De Wit E, Leach AR, Kortemme T, Shoichet B, Ott M, Saez-Rodriguez J, tenOever BR, Mullins RD, Fischer ER, Kochs G, Grosse R, García-Sastre A, Vignuzzi M, Johnson JR, Shokat KM, Swaney DL, Beltrao P, Krogan NJ
Cell 2020 Aug 6;182(3):685-712.e19
Cell 2020 Aug 6;182(3):685-712.e19
Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.
Kan AA, de Jager W, de Wit M, Heijnen C, van Zuiden M, Ferrier C, van Rijen P, Gosselaar P, Hessel E, van Nieuwenhuizen O, de Graan PN
Journal of neuroinflammation 2012 Aug 30;9:207
Journal of neuroinflammation 2012 Aug 30;9:207
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IL-22 by Western Blot analysis IL-22 Polyclonal Antibody, Biotin (Product # 500-P211BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible development reagents the detection limit for Recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IL-22 by Western Blot analysis IL-22 Polyclonal Antibody, Biotin (Product # 500-P211BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible development reagents the detection limit for Recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human IL-22 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of IL-22 Polyclonal Antibody, Biotin (Product # 500-P211BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-22 Polyclonal Antibody (Product # 500-P211-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-22.
- Conjugate
- Biotin
