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antibody from Novus Biologicals
Targeting: TLR2
CD282, TIL4
Western blot ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry
Flow cytometry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Flow cytometry [6]
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- Product number
- NB100-56726 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56726, RRID:AB_839176
- Product name
- Mouse Monoclonal TLR2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-κB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4.
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells.
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells.
Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4.
Cholesterol oxidase is indispensable in the pathogenesis of Mycobacterium tuberculosis.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis.
Cytoadherence-dependent induction of inflammatory responses by Mycoplasma pneumoniae.
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Early innate recognition of herpes simplex virus in human primary macrophages is mediated via the MDA5/MAVS-dependent and MDA5/MAVS/RNA polymerase III-independent pathways.
Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands.
Neisseria gonorrhoeae enhances HIV-1 infection of primary resting CD4+ T cells through TLR2 activation.
The Escherichia coli mqsR and ygiT genes encode a new toxin-antitoxin pair.
Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells.
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
The probiotic Escherichia coli strain Nissle 1917 induces gammadelta T cell apoptosis via caspase- and FasL-dependent pathways.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Signal transduction and nuclear responses in Staphylococcus aureus-induced expression of human beta-defensin 3 in skin keratinocytes.
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6.
A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6.
Expression and function of Toll-like receptor 2 in canine blood phagocytes.
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression.
Evidence of Toll-like receptor molecules on human platelets.
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice.
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells.
Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells.
Differential expression of Toll-like receptor 2 in human cells.
Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation.
Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation.
Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide.
Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide.
Ji X, Zhang X, Li H, Sun L, Hou X, Song H, Han L, Xu S, Qiu X, Wang X, Zheng N, Li Z
Infection and immunity 2020 Mar 23;88(4)
Infection and immunity 2020 Mar 23;88(4)
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells.
The E, Yao Q, Zhang P, Zhai Y, Ao L, Fullerton DA, Meng X
Cells 2020 Feb 7;9(2)
Cells 2020 Feb 7;9(2)
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells.
Li F, Song R, Ao L, Reece TB, Cleveland JC Jr, Dong N, Fullerton DA, Meng X
Arteriosclerosis, thrombosis, and vascular biology 2017 Jul;37(7):1339-1351
Arteriosclerosis, thrombosis, and vascular biology 2017 Jul;37(7):1339-1351
Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
Käbisch R, Mejías-Luque R, Gerhard M, Prinz C
PloS one 2014;9(8):e104804
PloS one 2014;9(8):e104804
Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4.
Rojas-Bernabé A, Garcia-Hernández O, Maldonado-Bernal C, Delegado-Dominguez J, Ortega E, Gutiérrez-Kobeh L, Becker I, Aguirre-Garcia M
Parasitology 2014 May;141(6):788-800
Parasitology 2014 May;141(6):788-800
Cholesterol oxidase is indispensable in the pathogenesis of Mycobacterium tuberculosis.
Klink M, Brzezinska M, Szulc I, Brzostek A, Kielbik M, Sulowska Z, Dziadek J
PloS one 2013;8(9):e73333
PloS one 2013;8(9):e73333
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
PloS one 2013;8(4):e60671
PloS one 2013;8(4):e60671
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis.
Brzezinska M, Szulc I, Brzostek A, Klink M, Kielbik M, Sulowska Z, Pawelczyk J, Dziadek J
BMC microbiology 2013 Feb 20;13:43
BMC microbiology 2013 Feb 20;13:43
Cytoadherence-dependent induction of inflammatory responses by Mycoplasma pneumoniae.
Shimizu T, Kida Y, Kuwano K
Immunology 2011 May;133(1):51-61
Immunology 2011 May;133(1):51-61
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Dasgupta G, Chentoufi AA, You S, Falatoonzadeh P, Urbano LA, Akhtarmalik A, Nguyen K, Ablabutyan L, Nesburn AB, BenMohamed L
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Early innate recognition of herpes simplex virus in human primary macrophages is mediated via the MDA5/MAVS-dependent and MDA5/MAVS/RNA polymerase III-independent pathways.
Melchjorsen J, Rintahaka J, Søby S, Horan KA, Poltajainen A, Østergaard L, Paludan SR, Matikainen S
Journal of virology 2010 Nov;84(21):11350-8
Journal of virology 2010 Nov;84(21):11350-8
Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands.
Oberg HH, Ly TT, Ussat S, Meyer T, Kabelitz D, Wesch D
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4733-40
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4733-40
Neisseria gonorrhoeae enhances HIV-1 infection of primary resting CD4+ T cells through TLR2 activation.
Ding J, Rapista A, Teleshova N, Mosoyan G, Jarvis GA, Klotman ME, Chang TL
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2814-24
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2814-24
The Escherichia coli mqsR and ygiT genes encode a new toxin-antitoxin pair.
Kasari V, Kurg K, Margus T, Tenson T, Kaldalu N
Journal of bacteriology 2010 Jun;192(11):2908-19
Journal of bacteriology 2010 Jun;192(11):2908-19
Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
Rydberg C, Månsson A, Uddman R, Riesbeck K, Cardell LO
Immunology 2009 Sep;128(1 Suppl):e600-11
Immunology 2009 Sep;128(1 Suppl):e600-11
Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells.
Ji S, Shin JE, Kim YS, Oh JE, Min BM, Choi Y
Infection and immunity 2009 Mar;77(3):1044-52
Infection and immunity 2009 Mar;77(3):1044-52
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
Yang X, Fullerton DA, Su X, Ao L, Cleveland JC Jr, Meng X
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
The probiotic Escherichia coli strain Nissle 1917 induces gammadelta T cell apoptosis via caspase- and FasL-dependent pathways.
Guzy C, Paclik D, Schirbel A, Sonnenborn U, Wiedenmann B, Sturm A
International immunology 2008 Jul;20(7):829-40
International immunology 2008 Jul;20(7):829-40
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
Prabha C, Rajashree P, Sulochana DD
Immunology letters 2008 Apr 15;117(1):26-34
Immunology letters 2008 Apr 15;117(1):26-34
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Signal transduction and nuclear responses in Staphylococcus aureus-induced expression of human beta-defensin 3 in skin keratinocytes.
Menzies BE, Kenoyer A
Infection and immunity 2006 Dec;74(12):6847-54
Infection and immunity 2006 Dec;74(12):6847-54
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, Koski G, Czerniecki BJ
Surgery 2006 Aug;140(2):170-8
Surgery 2006 Aug;140(2):170-8
A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6.
Shimizu T, Kida Y, Kuwano K
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4641-6
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4641-6
A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6.
Shimizu T, Kida Y, Kuwano K
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4641-6
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4641-6
Expression and function of Toll-like receptor 2 in canine blood phagocytes.
Bazzocchi C, Mortarino M, Comazzi S, Bandi C, Franceschi A, Genchi C
Veterinary immunology and immunopathology 2005 Mar 10;104(1-2):15-9
Veterinary immunology and immunopathology 2005 Mar 10;104(1-2):15-9
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Srivastava MD, Srivastava BI
Leukemia research 2005 Jul;29(7):813-20
Leukemia research 2005 Jul;29(7):813-20
Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression.
Zhong F, Cao W, Chan E, Tay PN, Cahya FF, Zhang H, Lu J
Immunology 2005 Jan;114(1):83-93
Immunology 2005 Jan;114(1):83-93
Evidence of Toll-like receptor molecules on human platelets.
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O
Immunology and cell biology 2005 Apr;83(2):196-8
Immunology and cell biology 2005 Apr;83(2):196-8
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, Laskay T
Journal of leukocyte biology 2004 Mar;75(3):467-77
Journal of leukocyte biology 2004 Mar;75(3):467-77
Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice.
Gibson FC 3rd, Hong C, Chou HH, Yumoto H, Chen J, Lien E, Wong J, Genco CA
Circulation 2004 Jun 8;109(22):2801-6
Circulation 2004 Jun 8;109(22):2801-6
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, Schnopp C, Fraunberger P, Walli AK, Ring J, Abeck D, Ollert M
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells.
Medvedev AE, Lentschat A, Wahl LM, Golenbock DT, Vogel SN
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5209-16
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5209-16
Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells.
Medvedev AE, Lentschat A, Wahl LM, Golenbock DT, Vogel SN
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5209-16
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5209-16
Differential expression of Toll-like receptor 2 in human cells.
Flo TH, Halaas O, Torp S, Ryan L, Lien E, Dybdahl B, Sundan A, Espevik T
Journal of leukocyte biology 2001 Mar;69(3):474-81
Journal of leukocyte biology 2001 Mar;69(3):474-81
Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation.
Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M
Journal of immunology (Baltimore, Md. : 1950) 2001 Feb 1;166(3):2018-24
Journal of immunology (Baltimore, Md. : 1950) 2001 Feb 1;166(3):2018-24
Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation.
Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M
Journal of immunology (Baltimore, Md. : 1950) 2001 Feb 1;166(3):2018-24
Journal of immunology (Baltimore, Md. : 1950) 2001 Feb 1;166(3):2018-24
Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide.
Flo TH, Halaas O, Lien E, Ryan L, Teti G, Golenbock DT, Sundan A, Espevik T
Journal of immunology (Baltimore, Md. : 1950) 2000 Feb 15;164(4):2064-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Feb 15;164(4):2064-9
Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide.
Flo TH, Halaas O, Lien E, Ryan L, Teti G, Golenbock DT, Sundan A, Espevik T
Journal of immunology (Baltimore, Md. : 1950) 2000 Feb 15;164(4):2064-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Feb 15;164(4):2064-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Intracellular analysis of TLR2 in 10^6 PBMs using 2 ug of TLR2 antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents TLR2 antibody. goat anti-mouse IgG FITC secondary antibody was used in this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using Azide/BSA FREE version of NB100-56726. Cell surface flow analysis of TLR2 in 10^6 PBMCs using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR2 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 ThP1 cells using 2 ug of this antibody. The closed histogram represents cells alone, green represents isotype control, red represents anti-TLR2 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR2 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 PBMCs (lymphocyte-gated) using 2 ugs ofMouse IgG2a Kappa , and red represents TLR2 antibody.
