Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB2616 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human TLR2 transfectants but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O60603
- Isotype
- IgG
- Antibody clone number
- 383936
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles.
Specific pathways mediating inflammasome activation by Candida parapsilosis.
CLEC5A is a critical receptor in innate immunity against Listeria infection.
A Model to Explain How the Bacille Calmette Guérin (BCG) Vaccine Drives Interleukin-12 Production in Neonates.
Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation.
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.
TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells.
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.
HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1α activation.
Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro.
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Sharma R, Ghasparian A, Robinson JA, McCullough KC
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):734-749
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):734-749
Specific pathways mediating inflammasome activation by Candida parapsilosis.
Tóth A, Zajta E, Csonka K, Vágvölgyi C, Netea MG, Gácser A
Scientific reports 2017 Feb 22;7:43129
Scientific reports 2017 Feb 22;7:43129
CLEC5A is a critical receptor in innate immunity against Listeria infection.
Chen ST, Li FJ, Hsu TY, Liang SM, Yeh YC, Liao WY, Chou TY, Chen NJ, Hsiao M, Yang WB, Hsieh SL
Nature communications 2017 Aug 21;8(1):299
Nature communications 2017 Aug 21;8(1):299
A Model to Explain How the Bacille Calmette Guérin (BCG) Vaccine Drives Interleukin-12 Production in Neonates.
Kativhu CL, Libraty DH
PloS one 2016;11(8):e0162148
PloS one 2016;11(8):e0162148
Vaccine-Mediated Activation of Human TLR4 Is Affected by Modulation of Culture Conditions during Whole-Cell Pertussis Vaccine Preparation.
Hoonakker ME, Verhagen LM, Pupo E, de Haan A, Metz B, Hendriksen CF, Han WG, Sloots A
PloS one 2016;11(8):e0161428
PloS one 2016;11(8):e0161428
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.
Smith IM, Baker A, Christensen JE, Boekhout T, Frøkiær H, Arneborg N, Jespersen L
PloS one 2016;11(11):e0167410
PloS one 2016;11(11):e0167410
TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells.
Ganesan S, Pham D, Jing Y, Farazuddin M, Hudy MH, Unger B, Comstock AT, Proud D, Lauring AS, Sajjan US
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2409-20
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 15;197(6):2409-20
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Vogel S, Börger V, Peters C, Förster M, Liebfried P, Metzger K, Meisel R, Däubener W, Trapp T, Fischer JC, Gawaz M, Sorg RV
Cell death and differentiation 2015 Jul;22(7):1219-30
Cell death and differentiation 2015 Jul;22(7):1219-30
Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.
Brummelman J, Veerman RE, Hamstra HJ, Deuss AJ, Schuijt TJ, Sloots A, Kuipers B, van Els CA, van der Ley P, Mooi FR, Han WG, Pinelli E
Infection and immunity 2015 Jan;83(1):227-38
Infection and immunity 2015 Jan;83(1):227-38
HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1α activation.
Park SY, Lee SW, Kim HY, Lee WS, Hong KW, Kim CD
European journal of immunology 2015 Apr;45(4):1216-27
European journal of immunology 2015 Apr;45(4):1216-27
Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
Lutay N, Håkansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
PloS one 2014;9(1):e86466
PloS one 2014;9(1):e86466
Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Müller I, Mack A, Schumann S, Bühring HJ, Lang F, Sorg RV, Langer H, Gawaz M
The Journal of biological chemistry 2014 Apr 18;289(16):11068-82
The Journal of biological chemistry 2014 Apr 18;289(16):11068-82
Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro.
Li D, Wang X, Wu JL, Quan WQ, Ma L, Yang F, Wu KY, Wan HY
PloS one 2013;8(2):e56616
PloS one 2013;8(2):e56616
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
Innate immunity 2012 Jun;18(3):531-40
Innate immunity 2012 Jun;18(3):531-40
Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
The British journal of dermatology 2011 Aug;165(2):302-10
The British journal of dermatology 2011 Aug;165(2):302-10
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
Clinical and experimental immunology 2009 May;156(2):285-93
Clinical and experimental immunology 2009 May;156(2):285-93
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TLR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-8 Secretion Induced by Pam3CSK4 and Neutralization by Human TLR2 Antibody. The synthetic tripalmitoylated lipopeptide Pam3CSK4 stimulates IL-8 secretion in the HEK293 human embryonic kidney cell line transfected with human TLR2, in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). IL-8 secretion elicited by Pam3CSK4 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TLR2 Monoclonal Antibody (Catalog # MAB2616). The ND50 is typically 0.03-0.15 µg/mL.