959-MSM1-P0

antibody from Invitrogen Antibodies

Targeting: CD40LG CD154, CD40L, gp39, hCD40L, HIGM1, IMD3, TNFSF5, TRAP

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

959-MSM1-P0

Provider

Invitrogen Antibodies

Product name

CD40 Ligand/CD154/TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (CD40LG, 2761)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CD40LG, 2761

Vial size

20 µg

Concentration

200 µg/mL

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed Jurkat cells using CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human spleen using CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human spleen using CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human tonsil using CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of Jurkat cells using CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDís) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDís) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE Analyis Purified CD40 Ligand, CD154, TRAP1 (Activation Marker of T-Lymphocytes) Monoclonal Antibody (Product # 959-MSM1-P1). Confirmation of Purity and Integrity of Antibody.