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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00003059-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003059-M01, RRID:AB_539460
- Product name
- Mouse Monoclonal HCLS1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HCLS1 - hematopoietic cell-specific Lyn substrate 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization.
Kan CY, Wen VW, Pasquier E, Jankowski K, Chang M, Richards LA, Kavallaris M, MacKenzie KL
Oncogene 2012 Nov 15;31(46):4815-27
Oncogene 2012 Nov 15;31(46):4815-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HCLS1 Antibody (3D5) [H00003059-M01] - HCLS1 monoclonal antibody (M01), clone 3D5 Analysis of HCLS1 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: HCLS1 Antibody (3D5) [H00003059-M01] - Detection limit for recombinant GST tagged HCLS1 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HCLS1 Antibody (3D5) [H00003059-M01] - Analysis of monoclonal antibody to HCLS1 on HeLa cell . Antibody concentration 10 ug/ml.
