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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-30057 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCLS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji.
- Concentration
- 0.95 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HCLS1 Polyclonal Antibody (Product # PA5-30057). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. HCLS1 antibody . HCLS1 Polyclonal Antibody (Product # PA5-30057) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 µg of HCLS1 antibody (Product # PA5-30057) 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody (Product # PA5-30057) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
