LS-C186218

antibody from LSBio

Targeting: PKLR

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C186218

Provider

LSBio

Product name

PKLR Antibody (aa1-260) LS-C186218

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A:293T, B: A431, C: HeLa, D: HepG2, E: A375. 7.5% SDS PAGE. PKLR antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis. A. 20 ug Rat liver lysate/extract. 7.5% SDS-PAGE. Pyruvate Kinase (liver/RBC) antibody dilution:1:10000.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis. HepG2 cells were fixed in -20 100% MeOH for 5 min. PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody diluted at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Pyruvate Kinase (liver/RBC) antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis. HepG2 cells were fixed in -20 100% MeOH for 5 min. PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded NCIN87 xenograft using Pyruvate Kinase (liver/RBC) antibody at 1:500 dilution.