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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
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- Product number
- PA5-34734 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKLR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562, Raw264.7, rat liver, human PKLR-transfected 293T. Predicted reactivity: Mouse (90%), Rat (90%), Dog (89%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.62 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKLR Polyclonal Antibody detects PKLR protein by Western blot analysis. A. 30 µg K562 whole cell lysate/extract.7.5 % SDS-PAGE. PKLR Polyclonal Antibody (Product # PA5-34734) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKLR Polyclonal Antibody detects PKLR protein by Western blot analysis. A. 30 µg Raw264.7 whole cell lysate/extract.7.5 % SDS-PAGE. PKLR Polyclonal Antibody (Product # PA5-34734) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKLR Polyclonal Antibody detects PKLR protein by Western blot analysis. A. 50 µg rat liver lysate/extract.7.5 % SDS-PAGE. PKLR Polyclonal Antibody (Product # PA5-34734) dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKLR Polyclonal Antibody detects Pyruvate Kinase (liver/RBC) protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 5 µg whole cell lysate/extract of human PKLR-transfected 293T cells.7.5 % SDS-PAGE. PKLR Polyclonal Antibody (Product # PA5-34734) dilution: 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PKLR Polyclonal Antibody (Product # PA5-34734) and bands between 58-62kDa band corresponding to PKLR was observed in K-562 and Hep G2, but not in U-2 OS, U-937 and Raji which are reported to be low expressing cell lines. Whole cell lysate (30ug lysate) of K-562 (Lane 1), Hep G2 (Lane 2), U-2 OS (Lane 3), U-937 (Lane 4) and Raji (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
