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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX83883 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83883, RRID:AB_10734276
- Product name
- Pyruvate Kinase (liver/RBC) antibody [1H4]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-PKLR mouse monoclonal antibody (GTX83883) immunofluorescent staining of COS7 cells transiently transfected with PKLR
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PKLR antibody(GTX83883),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206455 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PKLR antibody(GTX83883), and then analyzed by flow cytometry.
