Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501413 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501413, RRID:AB_11125785
- Product name
- PKLR mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
- Antibody type
- Monoclonal
- Description
- PKLR mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PKLR
- Isotype
- IgG
- Antibody clone number
- OTI1H4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PKLR mouse monoclonal antibody (TA501413) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206455 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PKLR antibody(TA501413), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PKLR antibody(TA501413),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
