TA501416

antibody from OriGene

Targeting: PKLR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501416

Provider

OriGene

Proper citation

OriGene Cat#TA501416, RRID:AB_11126945

Product name

Anti-PKLR mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)

Antibody type

Monoclonal

Description

Anti-PKLR mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)

Host

Mouse

Conjugate

Unconjugated

Epitope

PKLR

Isotype

IgG

Antibody clone number

OTI1H2

Vial size

100 µl

Concentration

0.78 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PKLR mouse monoclonal antibody (TA501416) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206455 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PKLR antibody(TA501416), and then analyzed by flow cytometry.

Validation comment

FC