PA5-79824

antibody from Invitrogen Antibodies

Targeting: PKLR

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79824

Provider

Invitrogen Antibodies

Product name

PKLR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Liver Pyruvate Kinase Promotes NAFLD/NASH in Both Mice and Humans in a Sex-Specific Manner.

Chella Krishnan K, Floyd RR, Sabir S, Jayasekera DW, Leon-Mimila PV, Jones AE, Cortez AA, Shravah V, Péterfy M, Stiles L, Canizales-Quinteros S, Divakaruni AS, Huertas-Vazquez A, Lusis AJ
Cellular and molecular gastroenterology and hepatology 2021;11(2):389-406

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 AAV-mediated modulation of hepatic L-PK expression. Eight-week-old male C57BL/6J mice were injected with either loss-of-function (shffLuc or shPklr) or gain-of-function (green fluorescent protein or PKLR) AAV vectors under the control of Tbg promoter and fed HF/HS diet for 17 additional weeks. ( A ) qPCR analyses of hepatic Pklr expression and ( B ) immunoblot analyses of hepatic PKLR in L-PK KD and OEx mice. GAPDH was used as a loading control. ( C ) Quantification of average PKLR levels normalized to GAPDH for each group. qPCR analyses of Pklr expression ( D ) 48 hours after siRNA transfection of AML12 cells (experiment was repeated 2 independent times with n = 3 wells per group each time) and ( E ) 12 weeks after NASH diet in L-PK KD mice. Data are presented as mean +- SEM (n = 6-8 livers for RNA and 3 for protein analyses per group). P values were calculated by unpaired Student t test. *** P < .001.