PA5-27305

antibody from Invitrogen Antibodies

Targeting: PKLR

Antibody data

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Validation data

Product number: PA5-27305 - Provider product page
Provider: Invitrogen Antibodies
Product name: PKLR Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Jurkat, Raji, K562, rat liver, PKLR-transfected 293T. Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (80%), Dog (93%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.01 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PKLR protein structure - PA5-27305 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PKLR was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKLR Polyclonal Antibody (Product # PA5-27305) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PKLR Polyclonal Antibody (Product # PA5-27305). Sample (30 µg of whole cell lysate). A: Jurkat. B: Raji. C: K562. 7.5% SDS PAGE. PKLR Polyclonal Antibody (Product # PA5-27305) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PKLR Polyclonal Antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis. A. 20 µg Rat liver lysate/extract. 7.5% SDS-PAGE. PKLR Polyclonal Antibody (Product # PA5-27305) dilution: 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-PKLR Polyclonal Antibody (Product # PA5-27305) and bands between 58-62kDa corresponding to PKLR was observed in K-562, Hep G2, U-2 OS and Raji, but not in U-937 which is reported to be negative. Whole cell lysate (30ug lysate) of K-562 (Lane 1), Hep G2 (Lane 2), U-2 OS (Lane 3), U-937 (Lane 4) and Raji (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).