Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-77709-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Acetylcholinesterase/ACHE Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography.
- Reactivity
- Bovine
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Acetylcholinesterase/ACHE Antibody [Biotin] [NBP1-77709] - Goat anti Pyranose Oxidase antibody was used to detect pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat 1:10K in TBS 1 hr RT.
