NBP2-22449

antibody from Novus Biologicals

Targeting: ACHE YT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Antibody Data

Product number

NBP2-22449

Provider

Novus Biologicals

Product name

Mouse Monoclonal Acetylcholinesterase/ACHE Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human, Mouse, Rat, Feline, Guinea Pig, Rabbit

Host

Mouse

Isotype

IgG

Vial size

100 uL

Concentration

1.7 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Western blot analysis was performed on whole cell extracts (30 ug lysate) of Mouse Brain (lane 1), HeLa (lane 2), SK-N-SH (lane 3) and U-87 MG (lane 4).

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) shows staining in U251 glioma cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) shows staining in C6 glioma cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Flow cytometry analysis of Acetyl cholinesterase was done on U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Acetyl cholinesterase Mouse Monoclonal Antibody (red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA.