HPA059353

antibody from Atlas Antibodies

Targeting: ZFHX3 ATBF1, C16orf47, FLJ26184, ZNF927

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA059353

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA059353, RRID:AB_2683987

Product name

Anti-ZFHX3

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ZFHX3, Gene description: zinc finger homeobox 3, Alternative Gene Names: ATBF1, ZNF927, Validated applications: IHC, ChIP, Uniprot ID: Q15911, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Haploinsufficiency of ZFHX3, encoding a key player in neuronal development, causes syndromic intellectual disability

Pérez Baca M, Jacobs E, Vantomme L, Leblanc P, Bogaert E, Dheedene A, De Cock L, Haghshenas S, Foroutan A, Levy M, Kerkhof J, McConkey H, Chen C, Batzir N, Wang X, Palomares M, Carels M, Agrawal P, Armstrong Scott D, Barkoudah E, Bellini M, Beneteau C, Bjørgo K, Brooks A, Brown N, Castle A, Castro D, Chorin O, Cleghorn M, Clement E, Coman D, Costin C, Devriendt K, Dong D, Dries A, Duelund Hjortshøj T, Dyment D, Eng C, Genetti C, Grano S, Henneman P, Heron D, Hoffmann K, Hom J, Du H, Iascone M, Isidor B, Järvelä I, Jones J, Keren B, Koenig M, Kohlhase J, Lalani S, Le Caignec C, Lewis A, Liu P, Lovgren A, Lupski J, Lyons M, Lysy P, Manning M, Marcelis C, McLean S, Mercie S, Mertens M, Molin A, Nizon M, Nugent K, Öhman S, O'Leary M, Okashah Littlejohn R, Petit F, Pfundt R, Pottocki L, Raas-Rotschild A, Ranguin K, Revencu N, Rosenfeld J, Rhodes L, Santos Simmaro F, Sals K, Schieving J, Schrauwen I, Schuurs-Hoeijmakers J, Seaby E, Sheffer R, Snijders Blok L, Sørensen K, Srivastava S, Stark Z, Stoeva R, Stutterd C, Tan N, Mathiesen Torring P, Vanakker O, van der Laan L, Ververi A, Villavicencio-Lorini P, Vincent M, Wand D, Wessels M, White S, Wojcik M, Wu N, Zhao S, Dermaut B, Sadikovic B, Menten B, Yuan B, Vergult S, Callewaert B
The American Journal of Human Genetics 2024;111(3):509-528

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-ZFHX3 (HPA059353, Lot 000023207) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.