Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [1]
 - ELISA [3]
 - Immunoprecipitation [3]
 
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Validation data
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- Product number
 - LS-C197785 - Provider product page

 - Provider
 - LSBio
 - Product name
 - NIT1 Antibody (clone 1C3) LS-C197785
 - Antibody type
 - Monoclonal
 - Description
 - Purified from ascites by Protein A
 - Reactivity
 - Human
 - Host
 - Mouse
 - Isotype
 - IgG
 - Antibody clone number
 - 1C3
 - Storage
 - Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
 
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					Enhanced validation
					
									
				
				- Submitted by
 - LSBio (provider)
 - Enhanced method
 - Genetic validation
 - Main image
 
- Experimental details
 - Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - LSBio (provider)
 - Enhanced method
 - Genetic validation
 - Main image
 
- Experimental details
 - Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
 
- Submitted by
 - LSBio (provider)
 - Main image
 
- Experimental details
 - Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
 
- Submitted by
 - LSBio (provider)
 - Main image
 
- Experimental details
 - Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - LSBio (provider)
 - Enhanced method
 - Genetic validation
 - Main image
 
- Experimental details
 - Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
 
- Submitted by
 - LSBio (provider)
 - Main image
 
- Experimental details
 - Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
 
- Submitted by
 - LSBio (provider)
 - Main image
 
- Experimental details
 - Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.