Antibody data
- Antibody Data
- Antigen structure
- References [33]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 11027-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11027-1-AP, RRID:AB_2165781
- Product name
- PLOD3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PLOD3 antibody (Cat. #11027-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes.
Cerebral small vessel disease caused by PLOD3 mutation: Expanding the phenotypic spectrum of lysyl hydroxylase-3 deficiency.
PLOD3 contributes to HER-2 therapy resistance in gastric cancer through FoxO3/Survivin pathway.
Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.
Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes.
PLOD3 Is Associated with Immune Cell Infiltration and Genomic Instability in Colon Adenocarcinoma.
Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.
A Human Pan-Cancer System Analysis of Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 3 (PLOD3).
p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.
Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1.
Identification and characterization of collagen-like glycosylation and hydroxylation of CCN1.
Overexpression of LH3 reduces the incidence of hypertensive intracerebral hemorrhage in mice.
Cyclophilin B control of lysine post-translational modifications of skin type I collagen.
Loss of function of Colgalt1 disrupts collagen post-translational modification and causes musculoskeletal defects.
PLOD3 suppression exerts an anti-tumor effect on human lung cancer cells by modulating the PKC-delta signaling pathway.
Role of Glycosyltransferase 25 Domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.
Mutations in PLOD3, encoding lysyl hydroxylase 3, cause a complex connective tissue disorder including recessive dystrophic epidermolysis bullosa-like blistering phenotype with abnormal anchoring fibrils and type VII collagen deficiency.
PLOD3 promotes lung metastasis via regulation of STAT3.
Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.
Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.
Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.
Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.
Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.
Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation.
Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.
Deregulation of the lysyl hydroxylase matrix cross-linking system in experimental and clinical bronchopulmonary dysplasia.
Lysyl hydroxylase 3 is secreted from cells by two pathways.
Proteomic dissection of the von Hippel-Lindau (VHL) interactome.
Characterisation of the Drosophila procollagen lysyl hydroxylase, dPlod.
Reduction of lysyl hydroxylase 3 causes deleterious changes in the deposition and organization of extracellular matrix.
The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability.
A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.
Wu SA, Shen C, Wei X, Zhang X, Wang S, Chen X, Torres M, Lu Y, Lin LL, Wang HH, Hunter AH, Fang D, Sun S, Ivanova MI, Lin Y, Qi L
Nature communications 2023 May 30;14(1):3132
Nature communications 2023 May 30;14(1):3132
Cerebral small vessel disease caused by PLOD3 mutation: Expanding the phenotypic spectrum of lysyl hydroxylase-3 deficiency.
Zhou J, Feng W, Zhuo X, Lu W, Wang J, Fang F, Wang X
Pediatric investigation 2022 Sep;6(3):219-223
Pediatric investigation 2022 Sep;6(3):219-223
PLOD3 contributes to HER-2 therapy resistance in gastric cancer through FoxO3/Survivin pathway.
Chen Y, Ye B, Wang C, Nie Y, Qin J, Shen Z
Cell death discovery 2022 Jul 14;8(1):321
Cell death discovery 2022 Jul 14;8(1):321
Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.
Saito T, Terajima M, Taga Y, Hayashi F, Oshima S, Kasamatsu A, Okubo Y, Ito C, Toshimori K, Sunohara M, Tanzawa H, Uzawa K, Yamauchi M
Bone 2022 Jan;154:116242
Bone 2022 Jan;154:116242
Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes.
Terajima M, Taga Y, Nakamura T, Guo HF, Kayashima Y, Maeda-Smithies N, Parag-Sharma K, Kim JS, Amelio AL, Mizuno K, Kurie JM, Yamauchi M
Scientific reports 2022 Aug 22;12(1):14256
Scientific reports 2022 Aug 22;12(1):14256
PLOD3 Is Associated with Immune Cell Infiltration and Genomic Instability in Colon Adenocarcinoma.
Deng X, Pan Y, Yang M, Liu Y, Li J
BioMed research international 2021;2021:4714526
BioMed research international 2021;2021:4714526
Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.
Jia W, Zou X, Xu Z, Bai L, Shan A, Li Y, Shi J, Yang F, Ding C, Narimatsu H, Zhang Y
Glycobiology 2021 Sep 9;31(8):947-958
Glycobiology 2021 Sep 9;31(8):947-958
A Human Pan-Cancer System Analysis of Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 3 (PLOD3).
Gong S, Duan Y, Wu C, Osterhoff G, Schopow N, Kallendrusch S
International journal of molecular sciences 2021 Sep 14;22(18)
International journal of molecular sciences 2021 Sep 14;22(18)
p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.
Tan X, Banerjee P, Shi L, Xiao GY, Rodriguez BL, Grzeskowiak CL, Liu X, Yu J, Gibbons DL, Russell WK, Creighton CJ, Kurie JM
Science advances 2021 Jun;7(25)
Science advances 2021 Jun;7(25)
Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1.
Mori K, Suzuki T, Miura K, Dohmae N, Simizu S
Biochemical and biophysical research communications 2021 Jun 30;560:93-98
Biochemical and biophysical research communications 2021 Jun 30;560:93-98
Identification and characterization of collagen-like glycosylation and hydroxylation of CCN1.
Ishizawa Y, Niwa Y, Suzuki T, Kawahara R, Dohmae N, Simizu S
Glycobiology 2019 Sep 20;29(10):696-704
Glycobiology 2019 Sep 20;29(10):696-704
Overexpression of LH3 reduces the incidence of hypertensive intracerebral hemorrhage in mice.
Li H, Xu H, Wen H, Liu T, Sun Y, Xiao N, Bai C, Ge J, Wang X, Song L, Song Y, Zhang Y, Chen J
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2019 Mar;39(3):547-561
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2019 Mar;39(3):547-561
Cyclophilin B control of lysine post-translational modifications of skin type I collagen.
Terajima M, Taga Y, Cabral WA, Liu Y, Nagasawa M, Sumida N, Kayashima Y, Chandrasekaran P, Han L, Maeda N, Perdivara I, Hattori S, Marini JC, Yamauchi M
PLoS genetics 2019 Jun;15(6):e1008196
PLoS genetics 2019 Jun;15(6):e1008196
Loss of function of Colgalt1 disrupts collagen post-translational modification and causes musculoskeletal defects.
Geister KA, Lopez-Jimenez AJ, Houghtaling S, Ho TH, Vanacore R, Beier DR
Disease models & mechanisms 2019 Jun 17;12(6)
Disease models & mechanisms 2019 Jun 17;12(6)
PLOD3 suppression exerts an anti-tumor effect on human lung cancer cells by modulating the PKC-delta signaling pathway.
Baek JH, Yun HS, Kwon GT, Lee J, Kim JY, Jo Y, Cho JM, Lee CW, Song JY, Ahn J, Kim JS, Kim EH, Hwang SG
Cell death & disease 2019 Feb 15;10(3):156
Cell death & disease 2019 Feb 15;10(3):156
Role of Glycosyltransferase 25 Domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.
Terajima M, Taga Y, Sricholpech M, Kayashima Y, Sumida N, Maeda N, Hattori S, Yamauchi M
Biochemistry 2019 Dec 17;58(50):5040-5051
Biochemistry 2019 Dec 17;58(50):5040-5051
Mutations in PLOD3, encoding lysyl hydroxylase 3, cause a complex connective tissue disorder including recessive dystrophic epidermolysis bullosa-like blistering phenotype with abnormal anchoring fibrils and type VII collagen deficiency.
Vahidnezhad H, Youssefian L, Saeidian AH, Touati A, Pajouhanfar S, Baghdadi T, Shadmehri AA, Giunta C, Kraenzlin M, Syx D, Malfait F, Has C, Lwin SM, Karamzadeh R, Liu L, Guy A, Hamid M, Kariminejad A, Zeinali S, McGrath JA, Uitto J
Matrix biology : journal of the International Society for Matrix Biology 2019 Aug;81:91-106
Matrix biology : journal of the International Society for Matrix Biology 2019 Aug;81:91-106
PLOD3 promotes lung metastasis via regulation of STAT3.
Baek JH, Yun HS, Kwon GT, Kim JY, Lee CW, Song JY, Um HD, Kang CM, Park JK, Kim JS, Kim EH, Hwang SG
Cell death & disease 2018 Nov 15;9(12):1138
Cell death & disease 2018 Nov 15;9(12):1138
Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.
Scietti L, Chiapparino A, De Giorgi F, Fumagalli M, Khoriauli L, Nergadze S, Basu S, Olieric V, Cucca L, Banushi B, Profumo A, Giulotto E, Gissen P, Forneris F
Nature communications 2018 Aug 8;9(1):3163
Nature communications 2018 Aug 8;9(1):3163
Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.
Cowling RT, Park JI, Sotimehin AE, Greenberg BH
Journal of molecular and cellular cardiology 2017 Dec;113:1-8
Journal of molecular and cellular cardiology 2017 Dec;113:1-8
Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.
Yun HS, Baek JH, Yim JH, Um HD, Park JK, Song JY, Park IC, Kim JS, Lee SJ, Lee CW, Hwang SG
Cancer biology & therapy 2016;17(2):208-18
Cancer biology & therapy 2016;17(2):208-18
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues.
Ohe R, Aung NY, Meng H, Kabasawa T, Suto A, Tamazawa N, Yang S, Kato T, Yamakawa M
Leukemia & lymphoma 2016 Jul;57(7):1687-96
Leukemia & lymphoma 2016 Jul;57(7):1687-96
Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.
Banushi B, Forneris F, Straatman-Iwanowska A, Strange A, Lyne AM, Rogerson C, Burden JJ, Heywood WE, Hanley J, Doykov I, Straatman KR, Smith H, Bem D, Kriston-Vizi J, Ariceta G, Risteli M, Wang C, Ardill RE, Zaniew M, Latka-Grot J, Waddington SN, Howe SJ, Ferraro F, Gjinovci A, Lawrence S, Marsh M, Girolami M, Bozec L, Mills K, Gissen P
Nature communications 2016 Jul 20;7:12111
Nature communications 2016 Jul 20;7:12111
Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.
Watt SA, Dayal JH, Wright S, Riddle M, Pourreyron C, McMillan JR, Kimble RM, Prisco M, Gartner U, Warbrick E, McLean WH, Leigh IM, McGrath JA, Salas-Alanis JC, Tolar J, South AP
PloS one 2015;10(9):e0137639
PloS one 2015;10(9):e0137639
Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation.
Dayer C, Stamenkovic I
The Journal of biological chemistry 2015 May 29;290(22):13763-78
The Journal of biological chemistry 2015 May 29;290(22):13763-78
Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.
Cabral WA, Perdivara I, Weis M, Terajima M, Blissett AR, Chang W, Perosky JE, Makareeva EN, Mertz EL, Leikin S, Tomer KB, Kozloff KM, Eyre DR, Yamauchi M, Marini JC
PLoS genetics 2014 Jun;10(6):e1004465
PLoS genetics 2014 Jun;10(6):e1004465
Deregulation of the lysyl hydroxylase matrix cross-linking system in experimental and clinical bronchopulmonary dysplasia.
Witsch TJ, Turowski P, Sakkas E, Niess G, Becker S, Herold S, Mayer K, Vadász I, Roberts JD Jr, Seeger W, Morty RE
American journal of physiology. Lung cellular and molecular physiology 2014 Feb;306(3):L246-59
American journal of physiology. Lung cellular and molecular physiology 2014 Feb;306(3):L246-59
Lysyl hydroxylase 3 is secreted from cells by two pathways.
Wang C, Ristiluoma MM, Salo AM, Eskelinen S, Myllylä R
Journal of cellular physiology 2012 Feb;227(2):668-75
Journal of cellular physiology 2012 Feb;227(2):668-75
Proteomic dissection of the von Hippel-Lindau (VHL) interactome.
Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y
Journal of proteome research 2011 Nov 4;10(11):5175-82
Journal of proteome research 2011 Nov 4;10(11):5175-82
Characterisation of the Drosophila procollagen lysyl hydroxylase, dPlod.
Bunt S, Denholm B, Skaer H
Gene expression patterns : GEP 2011 Jan-Feb;11(1-2):72-8
Gene expression patterns : GEP 2011 Jan-Feb;11(1-2):72-8
Reduction of lysyl hydroxylase 3 causes deleterious changes in the deposition and organization of extracellular matrix.
Risteli M, Ruotsalainen H, Salo AM, Sormunen R, Sipilä L, Baker NL, Lamandé SR, Vimpari-Kauppinen L, Myllylä R
The Journal of biological chemistry 2009 Oct 9;284(41):28204-28211
The Journal of biological chemistry 2009 Oct 9;284(41):28204-28211
The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability.
Wang C, Kovanen V, Raudasoja P, Eskelinen S, Pospiech H, Myllylä R
Journal of cellular and molecular medicine 2009 Mar;13(3):508-21
Journal of cellular and molecular medicine 2009 Mar;13(3):508-21
A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.
Salo AM, Cox H, Farndon P, Moss C, Grindulis H, Risteli M, Robins SP, Myllylä R
American journal of human genetics 2008 Oct;83(4):495-503
American journal of human genetics 2008 Oct;83(4):495-503
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP(PLOD3 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The PLOD3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PLOD3. This antibody recognizes human,mouse,rat antigen. The PLOD3 antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-PLOD3 (IP:11027-1-AP, 3ug; Detection:11027-1-AP 1:500) with HepG2 cells lysate 6000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 11027-1-AP(PLOD3 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue