60058-1-IG

antibody from Invitrogen Antibodies

Targeting: PLOD3 LH3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

60058-1-IG

Provider

Invitrogen Antibodies

Product name

PLOD3 Monoclonal Antibody (2G12B8)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: HEPHIADSW PQLQDHFSAV KLVGPEEALS PGEARDMAMD LCRQDPECEF YFSLDADAVL TNLQTLRILI EENRKVIAPM LSRHGKLWSN FWGALSPDEY YARSEDYVEL VQRKRVGVWN VPYISQAYVI RGDTLRMELP QRDVFSGSDT DPDMAFCKSF RDKGIFLHLS NQHEFGRLLA TSRYDTEHLH PDLWQIFDNP VDWKEQYIHE NYSRALEGEG IVEQPCPDVY WFPLLSEQMC DELVAEMEHY GQWSGGRHED SRLAGGYENV PTVDIHMKQV GYEDQWLQLL RTYVGPMTES LFPGYHTKAR AVMNFVVRYR PDEQPSLRPH HDSSTFTLNV ALNHKGLDYE GGGCRFLRYD CVISSPRKGW ALLHPGRLTH YHEGLPTTWG TRYIMVSFVD P (339-738 aa encoded by BC011674)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2G12B8

Vial size

150 µL

Concentration

1.6 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-IG (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PLOD3 antibody (60058-1-IG, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using (Product # 60058-1-IG) (PLOD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 60058-1-IG (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 60058-1-IG (PLOD3 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 60058-1-IG (PLOD3 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PLOD3 (IP:60058-1-IG, 5ug; Detection:60058-1-IG 1:500) with HeLa cells lysate 1400ug.