Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS7187 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PAK4 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length Human PAK4.
- Description
- Three recently identified isoforms of serine/threonine kinases, designated Ï«PAK p68, ÉÇPAK p65 and ÎÛPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The Ï«, ÉÇ and ÎÛPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot analysis of PAK4 polyclonal antibody