AM20217PU-N

antibody from Acris Antibodies GmbH

Targeting: PAK4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20217PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20217PU-N, RRID:AB_10754577

Product name

anti PAK4 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to hemocyaninEpitope: Peptide derived from the kinase activation loop.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

6C1

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous PAK4: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20217PU-N PAK4 antibody (Clone 6C1) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: A431 Lane 2:SW480 Lane 3: SW620Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D