GTX11428

antibody from GeneTex

Targeting: S100A1 S100-alpha, S100A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX11428

Provider

GeneTex

Proper citation

GeneTex Cat#GTX11428, RRID:AB_372948

Product name

S100 alpha antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Rabbit

References

Submitted references

Lack of S100A1 in mice confers a gender-dependent hypertensive phenotype and increased mortality after myocardial infarction.

Desjardins JF, Pourdjabbar A, Quan A, Leong-Poi H, Teichert-Kuliszewska K, Verma S, Parker TG
American journal of physiology. Heart and circulatory physiology 2009 May;296(5):H1457-65

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of S100-alpha from human muscle extract using GTX11428.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of S100 alpha (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a S100 alpha polyclonal antibody (GTX11428) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjμgated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.