GTX28330

antibody from GeneTex

Targeting: S100A1 S100-alpha, S100A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX28330

Provider

GeneTex

Proper citation

GeneTex Cat#GTX28330, RRID:AB_368582

Product name

S100 antibody [8B10]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Storage

Keep as concentrated solution, aliquot and store at 4¢XC. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Interaction of several monoclonal antibodies available to the S100 protein (1 g) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = GTX14849, Lane 2 = GTX28330, ab Lane 3 = GTX10203, Lane 4 = GTX28334

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

S-100 calibration curve, from one step assay in streptavidin coated plates. GTX28330 [200 ng/well] was used as capture and Eu- labelled GTX10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20C.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX28330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 20x objective