Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX28330 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX28330, RRID:AB_368582
- Product name
- S100 antibody [8B10]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- Keep as concentrated solution, aliquot and store at 4¢XC. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Interaction of several monoclonal antibodies available to the S100 protein (1 g) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = GTX14849, Lane 2 = GTX28330, ab Lane 3 = GTX10203, Lane 4 = GTX28334
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- S-100 calibration curve, from one step assay in streptavidin coated plates. GTX28330 [200 ng/well] was used as capture and Eu- labelled GTX10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20C.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX28330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 20x objective