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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA801079 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA801079, RRID:AB_2625793
- Product name
- S100A1 (S100 alpha) mouse monoclonal antibody, clone OTI9F3 (formerly 9F3)
- Antibody type
- Monoclonal
- Description
- S100A1 (S100 alpha) mouse monoclonal antibody, clone OTI9F3 (formerly 9F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- S100A1
- Isotype
- IgG
- Antibody clone number
- OTI9F3
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Standard curve for ELISA analysis with S100A1 recombinant protein (dilution range from 100pg/ml to 1ug/ml) using S100A1 Capture Antibody (Cat# TA801079) at 5ug/ml and HRP conjugated S100A1 Detection mAb (Cat# TA800811) at 0.033ug/ml.
- Validation comment
- ELISA
