Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4476 - Provider product page
- Provider
- R&D Systems
- Product name
- Human S100A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human S100A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) S100B, rhS100P, and rhS100A2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P23297
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references S100A1 is released from ischemic cardiomyocytes and signals myocardial damage via Toll-like receptor 4.
S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.
Rohde D, Schön C, Boerries M, Didrihsone I, Ritterhoff J, Kubatzky KF, Völkers M, Herzog N, Mähler M, Tsoporis JN, Parker TG, Linke B, Giannitsis E, Gao E, Peppel K, Katus HA, Most P
EMBO molecular medicine 2014 Jun;6(6):778-94
EMBO molecular medicine 2014 Jun;6(6):778-94
S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.
Yamaguchi F, Umeda Y, Shimamoto S, Tsuchiya M, Tokumitsu H, Tokuda M, Kobayashi R
The Journal of biological chemistry 2012 Apr 20;287(17):13787-98
The Journal of biological chemistry 2012 Apr 20;287(17):13787-98
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human S100A1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4476) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.