Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-83341 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83341, RRID:AB_11021957
- Product name
- Rabbit Polyclonal MRGPRF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MRGPRF antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: MRGPRF Antibody [NBP1-83341] - Analysis in control (vector only transfected HEK293T lysate) and MRGPRF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human heart muscle shows moderate positivity of the nuclear membrane in myocytes.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry: MRGPRF Antibody [NBP1-83341] - Staining of human endometrium shows weak to moderate membranous positivity in stromal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Analysis in human prostate and skeletal muscle tissues. Corresponding MRGPRF RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human skeletal muscle shows no positivity in myocytes as expected.