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- Product number
- PA1-934A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calcium Sensing Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-934A detects calcium sensing receptor (CaSR) from human and rat tissues. PA1-934A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~120 kDa protein representing CaSR from HEK393 cells and rat brain homogenate. The PA1-934A immunizing peptide corresponds to amino acid residues 12-27 from rat CaSR. This peptide (Cat.# PEP-009) is available for use in neutralization and control experiments.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Calcium-Sensing Receptor Contributes to Hyperoxia Effects on Human Fetal Airway Smooth Muscle.
Calcium sensing receptor in developing human airway smooth muscle.
Evidence for a regulated Ca(2+) entry in proximal tubular cells and its implication in calcium stone formation.
Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study.
Decreased parathyroid Klotho expression is associated with persistent hyperparathyroidism after kidney transplantation.
A novel germline inactivating mutation in the CASR gene in an Italian kindred affected by familial hypocalciuric hypercalcemia.
In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.
Stimulation of the calcium-sensing receptor stabilizes the podocyte cytoskeleton, improves cell survival, and reduces toxin-induced glomerulosclerosis.
Calcium-dependent diuretic system in preascitic liver cirrhosis.
Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.
Regulation of mouse lung development by the extracellular calcium-sensing receptor, CaR.
Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands.
The calcium sensing receptor and its alternatively spliced form in keratinocyte differentiation.
Roesler AM, Ravix J, Bartman CM, Patel BS, Schiliro M, Roos B, Nesbitt L, Pabelick CM, Martin RJ, MacFarlane PM, Prakash YS
Frontiers in physiology 2021;12:585895
Frontiers in physiology 2021;12:585895
Calcium sensing receptor in developing human airway smooth muscle.
Roesler AM, Wicher SA, Ravix J, Britt RD Jr, Manlove L, Teske JJ, Cummings K, Thompson MA, Farver C, MacFarlane P, Pabelick CM, Prakash YS
Journal of cellular physiology 2019 Aug;234(8):14187-14197
Journal of cellular physiology 2019 Aug;234(8):14187-14197
Evidence for a regulated Ca(2+) entry in proximal tubular cells and its implication in calcium stone formation.
Ibeh CL, Yiu AJ, Kanaras YL, Paal E, Birnbaumer L, Jose PA, Bandyopadhyay BC
Journal of cell science 2019 Apr 30;132(9)
Journal of cell science 2019 Apr 30;132(9)
Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study.
Baio G, Rescinito G, Rosa F, Pace D, Boccardo S, Basso L, Salvi S, Calabrese M, Truini M, Neumaier CE
Molecular imaging and biology 2015 Aug;17(4):548-56
Molecular imaging and biology 2015 Aug;17(4):548-56
Decreased parathyroid Klotho expression is associated with persistent hyperparathyroidism after kidney transplantation.
Hong YA, Choi DE, Lim SW, Yang CW, Chang YK
Transplantation proceedings 2013 Oct;45(8):2957-62
Transplantation proceedings 2013 Oct;45(8):2957-62
A novel germline inactivating mutation in the CASR gene in an Italian kindred affected by familial hypocalciuric hypercalcemia.
Falchetti A, Gozzini A, Terranegra A, Soldati L, Vezzoli G, Leoncini G, Giusti F, Franceschelli F, Masi L, Tanini A, Cavalli L, Brandi ML
European journal of endocrinology 2012 May;166(5):933-40
European journal of endocrinology 2012 May;166(5):933-40
In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.
Baio G, Fabbi M, Emionite L, Cilli M, Salvi S, Ghedin P, Prato S, Carbotti G, Tagliafico A, Truini M, Neumaier CE
European radiology 2012 Mar;22(3):551-8
European radiology 2012 Mar;22(3):551-8
Stimulation of the calcium-sensing receptor stabilizes the podocyte cytoskeleton, improves cell survival, and reduces toxin-induced glomerulosclerosis.
Oh J, Beckmann J, Bloch J, Hettgen V, Mueller J, Li L, Hoemme M, Gross ML, Penzel R, Mundel P, Schaefer F, Schmitt CP
Kidney international 2011 Sep;80(5):483-92
Kidney international 2011 Sep;80(5):483-92
Calcium-dependent diuretic system in preascitic liver cirrhosis.
Sansoè G, Aragno M, Tomasinelli CE, di Bonzo LV, Wong F, Parola M
Journal of hepatology 2010 Nov;53(5):856-62
Journal of hepatology 2010 Nov;53(5):856-62
Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.
Ishikawa M, Kimura K, Tachibana T, Hashimoto H, Shimojo M, Ueshiba H, Tsuboi K, Shibuya K, Yoshino G
Human cell 2010 May;23(2):58-64
Human cell 2010 May;23(2):58-64
Regulation of mouse lung development by the extracellular calcium-sensing receptor, CaR.
Finney BA, del Moral PM, Wilkinson WJ, Cayzac S, Cole M, Warburton D, Kemp PJ, Riccardi D
The Journal of physiology 2008 Dec 15;586(24):6007-19
The Journal of physiology 2008 Dec 15;586(24):6007-19
Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands.
Yang AH, Hsu CW, Chen JY, Tseng LM, Won GS, Lee CH
Virchows Archiv : an international journal of pathology 2006 Jul;449(1):62-8
Virchows Archiv : an international journal of pathology 2006 Jul;449(1):62-8
The calcium sensing receptor and its alternatively spliced form in keratinocyte differentiation.
Oda Y, Tu CL, Pillai S, Bikle DD
The Journal of biological chemistry 1998 Sep 4;273(36):23344-52
The Journal of biological chemistry 1998 Sep 4;273(36):23344-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of PC12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Hyperoxia effect on expression of the extracellular Ca 2+ ([Ca 2+ ] o ) sensing receptor (CaSR) in developing human fetal (
