Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA328603 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Calcium Sensing Receptor (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Calcium Sensing Receptor (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CASR
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Colo205 (lanes 1 and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates: 1,2,5. Anti-Calcium Sensing Receptor (extracellular) antibody (1:200). 3,4,6. Anti-Calcium Sensing Receptor (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of CaSR in C6 cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized C6 (rat brain glioma) cells. A. Staining using Anti-Calcium Sensing Receptor (extracellular) antibody(1:50), followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using Hoechst 33342. C. Merged images of panels A and B.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect Flow cytometry analysis of live Jurkat (human acute T cell leukemia) cells: black line, Unstained cells. green line, Cells + Anti-Calcium Sensing Receptor (extracellular) antibody , (10 ug/5x105 cells).
- Validation comment
- FC