NB120-19347

Nery FC, Siranosian JJ, Rosales I, Deguise MO, Sharma A, Muhtaseb AW, Nwe P, Johnstone AJ, Zhang R, Fatouraei M, Huemer N, Alves CRR, Kothary R, Swoboda KJ
Neurology. Genetics 2019 Oct;5(5):e353

Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear.

Bächinger D, Egli H, Goosmann MM, Monge Naldi A, Eckhard AH
Cell and tissue research 2019 Nov;378(2):163-173

Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro.

Luchsinger LL, Strikoudis A, Danzl NM, Bush EC, Finlayson MO, Satwani P, Sykes M, Yazawa M, Snoeck HW
Cell stem cell 2019 Aug 1;25(2):225-240.e7

Activation of the calcium-sensing receptor in human valvular interstitial cells promotes calcification.

Issa H, Hénaut L, Abdallah JB, Boudot C, Lenglet G, Avondo C, Ibrik A, Caus T, Brazier M, Mentaverri R, Zibara K, Kamel S
Journal of molecular and cellular cardiology 2019 Apr;129:2-12

Calcium-sensing receptor (CaSR) promotes development of bone metastasis in renal cell carcinoma.

Frees S, Breuksch I, Haber T, Bauer HK, Chavez-Munoz C, Raven P, Moskalev I, D Costa N, Tan Z, Daugaard M, Thüroff JW, Haferkamp A, Prawitt D, So A, Brenner W
Oncotarget 2018 Mar 20;9(21):15766-15779

External Ca²⁺ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells.

Dai XQ, Perez PL, Soria G, Scarinci N, Smoler M, Morsucci DC, Suzuki K, Cantero MDR, Cantiello HF
Experimental cell research 2017 Jan 1;350(1):50-61

Overexpression of a functional calcium-sensing receptor dramatically increases osteolytic potential of MDA-MB-231 cells in a mouse model of bone metastasis through epiregulin-mediated osteoprotegerin downregulation.

Boudot C, Hénaut L, Thiem U, Geraci S, Galante M, Saldanha P, Saidak Z, Six I, Clézardin P, Kamel S, Mentaverri R
Oncotarget 2017 Aug 22;8(34):56460-56472

A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors.

Koh J, Hogue JA, Sosa JA
PloS one 2016;11(8):e0161134

Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism.

Koh J, Hogue JA, Wang Y, DiSalvo M, Allbritton NL, Shi Y, Olson JA Jr, Sosa JA
Journal of cellular and molecular medicine 2016 Feb;20(2):351-9

Parathyroid-specific deletion of dicer-dependent microRNAs abrogates the response of the parathyroid to acute and chronic hypocalcemia and uremia.

Shilo V, Ben-Dov IZ, Nechama M, Silver J, Naveh-Many T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Sep;29(9):3964-76

Calcium-sensing receptor sequencing in 21 patients with idiopathic or familial parathyroid disorder: pitfalls and characterization of a novel I32 V loss-of-function mutation.

Szalat A, Shahar M, Shpitzen S, Nachmias B, Munter G, Gillis D, Durst R, Mevorach D, Leitersdorf E, Meiner V, Rosen H
Endocrine 2015 Mar;48(2):444-53

Parathyroid-specific deletion of Klotho unravels a novel calcineurin-dependent FGF23 signaling pathway that regulates PTH secretion.

Olauson H, Lindberg K, Amin R, Sato T, Jia T, Goetz R, Mohammadi M, Andersson G, Lanske B, Larsson TE
PLoS genetics 2013;9(12):e1003975

Pharmacology of AMG 416 (Velcalcetide), a novel peptide agonist of the calcium-sensing receptor, for the treatment of secondary hyperparathyroidism in hemodialysis patients.

Walter S, Baruch A, Dong J, Tomlinson JE, Alexander ST, Janes J, Hunter T, Yin Q, Maclean D, Bell G, Mendel DB, Johnson RM, Karim F
The Journal of pharmacology and experimental therapeutics 2013 Aug;346(2):229-40

Deficiency of the calcium-sensing receptor in the kidney causes parathyroid hormone-independent hypocalciuria.

Toka HR, Al-Romaih K, Koshy JM, DiBartolo S 3rd, Kos CH, Quinn SJ, Curhan GC, Mount DB, Brown EM, Pollak MR
Journal of the American Society of Nephrology : JASN 2012 Nov;23(11):1879-90

Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease.

Galitzer H, Ben-Dov IZ, Silver J, Naveh-Many T
Kidney international 2010 Feb;77(3):211-8

Deletion of the vitamin D receptor specifically in the parathyroid demonstrates a limited role for the receptor in parathyroid physiology.

Meir T, Levi R, Lieben L, Libutti S, Carmeliet G, Bouillon R, Silver J, Naveh-Many T
American journal of physiology. Renal physiology 2009 Nov;297(5):F1192-8

Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells.

Alam MU, Kirton JP, Wilkinson FL, Towers E, Sinha S, Rouhi M, Vizard TN, Sage AP, Martin D, Ward DT, Alexander MY, Riccardi D, Canfield AE
Cardiovascular research 2009 Feb 1;81(2):260-8

Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231.

Weston AH, Absi M, Ward DT, Ohanian J, Dodd RH, Dauban P, Petrel C, Ruat M, Edwards G
Circulation research 2005 Aug 19;97(4):391-8

Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor.

Goldsmith PK, Fan GF, Ray K, Shiloach J, McPhie P, Rogers KV, Spiegel AM
The Journal of biological chemistry 1999 Apr 16;274(16):11303-9

Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor.

Goldsmith PK, Fan GF, Ray K, Shiloach J, McPhie P, Rogers KV, Spiegel AM
The Journal of biological chemistry 1999 Apr 16;274(16):11303-9

Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition.

Goldsmith PK, Fan G, Miller JL, Rogers KV, Spiegel AM
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1997 Nov;12(11):1780-8

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of calcium sensing receptor in HEK7-2 cell extract.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CaSR in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of mouse stomach stained with Calcium Sensing Receptor Monoclonal Antibody. Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at 4C in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8um cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4C, immersing in sodium citrate buffer for 10 minutes at 100C before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with MA1-934 (diluted 1:100 in blocking buffer) overnight at 4C followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT. Sections were also stained with DAPI nuclear stain (blue). NB120-19347 positive cells (green) were found at the base of the antral glands in the mouse stomach. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human brain tissue.