ABIN266769

antibody from antibodies-online

Targeting: CASR FHH, GPRC2A, HHC, HHC1, NSHPT

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ABIN266769

Provider

antibodies-online

Product name

anti-Calcium-Sensing Receptor (CASR) antibody

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

5C10-ADD

Vial size

0.1 mg

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Handling

Avoid freeze-thaw cycles

References

Submitted references

External Ca²⁺ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells.

Dai XQ, Perez PL, Soria G, Scarinci N, Smoler M, Morsucci DC, Suzuki K, Cantero MDR, Cantiello HF
Experimental cell research 2017 Jan 1;350(1):50-61

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Overexpression of a functional calcium-sensing receptor dramatically increases osteolytic potential of MDA-MB-231 cells in a mouse model of bone metastasis through epiregulin-mediated osteoprotegerin downregulation.

Boudot C, Hénaut L, Thiem U, Geraci S, Galante M, Saldanha P, Saidak Z, Six I, Clézardin P, Kamel S, Mentaverri R
Oncotarget 2017 Aug 22;8(34):56460-56472

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A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors.

Koh J, Hogue JA, Sosa JA
PloS one 2016;11(8):e0161134

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Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism.

Koh J, Hogue JA, Wang Y, DiSalvo M, Allbritton NL, Shi Y, Olson JA Jr, Sosa JA
Journal of cellular and molecular medicine 2016 Feb;20(2):351-9

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Parathyroid-specific deletion of dicer-dependent microRNAs abrogates the response of the parathyroid to acute and chronic hypocalcemia and uremia.

Shilo V, Ben-Dov IZ, Nechama M, Silver J, Naveh-Many T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Sep;29(9):3964-76

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Calcium-sensing receptor sequencing in 21 patients with idiopathic or familial parathyroid disorder: pitfalls and characterization of a novel I32 V loss-of-function mutation.

Szalat A, Shahar M, Shpitzen S, Nachmias B, Munter G, Gillis D, Durst R, Mevorach D, Leitersdorf E, Meiner V, Rosen H
Endocrine 2015 Mar;48(2):444-53

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Parathyroid-specific deletion of Klotho unravels a novel calcineurin-dependent FGF23 signaling pathway that regulates PTH secretion.

Olauson H, Lindberg K, Amin R, Sato T, Jia T, Goetz R, Mohammadi M, Andersson G, Lanske B, Larsson TE
PLoS genetics 2013;9(12):e1003975

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Pharmacology of AMG 416 (Velcalcetide), a novel peptide agonist of the calcium-sensing receptor, for the treatment of secondary hyperparathyroidism in hemodialysis patients.

Walter S, Baruch A, Dong J, Tomlinson JE, Alexander ST, Janes J, Hunter T, Yin Q, Maclean D, Bell G, Mendel DB, Johnson RM, Karim F
The Journal of pharmacology and experimental therapeutics 2013 Aug;346(2):229-40

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Deficiency of the calcium-sensing receptor in the kidney causes parathyroid hormone-independent hypocalciuria.

Toka HR, Al-Romaih K, Koshy JM, DiBartolo S 3rd, Kos CH, Quinn SJ, Curhan GC, Mount DB, Brown EM, Pollak MR
Journal of the American Society of Nephrology : JASN 2012 Nov;23(11):1879-90

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Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease.

Galitzer H, Ben-Dov IZ, Silver J, Naveh-Many T
Kidney international 2010 Feb;77(3):211-8

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Deletion of the vitamin D receptor specifically in the parathyroid demonstrates a limited role for the receptor in parathyroid physiology.

Meir T, Levi R, Lieben L, Libutti S, Carmeliet G, Bouillon R, Silver J, Naveh-Many T
American journal of physiology. Renal physiology 2009 Nov;297(5):F1192-8

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Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells.

Alam MU, Kirton JP, Wilkinson FL, Towers E, Sinha S, Rouhi M, Vizard TN, Sage AP, Martin D, Ward DT, Alexander MY, Riccardi D, Canfield AE
Cardiovascular research 2009 Feb 1;81(2):260-8

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Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231.

Weston AH, Absi M, Ward DT, Ohanian J, Dodd RH, Dauban P, Petrel C, Ruat M, Edwards G
Circulation research 2005 Aug 19;97(4):391-8

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Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor.

Goldsmith PK, Fan GF, Ray K, Shiloach J, McPhie P, Rogers KV, Spiegel AM
The Journal of biological chemistry 1999 Apr 16;274(16):11303-9

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Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition.

Goldsmith PK, Fan G, Miller JL, Rogers KV, Spiegel AM
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1997 Nov;12(11):1780-8

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC