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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- RQ4484 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CASR Antibody / Calcium Sensing Receptor
- Antibody type
- Monoclonal
- Description
- This highly specific CASR antibody is suitable for use in Western blot applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 11E9.
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CASR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) A549, 3) 22RV1, 4) HepG2 and 5) Caco-2 lysate with CASR antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa, but is routinely observed at up to ~160 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) A549, 3) 22RV1, 4) HepG2 and 5) Caco-2 lysate with CASR antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa, but is routinely observed at up to ~160 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse Neuro-2a cells with CASR antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CASR antibody.
