MA1-16940

antibody from Invitrogen Antibodies

Targeting: CASR FHH, GPRC2A, HHC, HHC1, NSHPT

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Radioimmunoassay (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16940

Provider

Invitrogen Antibodies

Product name

Calcium Sensing Receptor Monoclonal Antibody (HL 1499)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: antigen standard for CASR (transient overexpression lysate), human kidney protein.

Reactivity

Human, Mouse, Rat, Rabbit

Host

Mouse

Isotype

IgG

Antibody clone number

HL 1499

Vial size

100 μL

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Calcium-Sensing Receptor Is Functionally Expressed in the Cochlear Perilymphatic Compartment and Essential for Hearing.

Minakata T, Inagaki A, Yamamura A, Yamamura H, Sekiya S, Murakami S
Frontiers in molecular neuroscience 2019;12:175

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Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice.

Laporta J, Keil KP, Vezina CM, Hernandez LL
PloS one 2014;9(10):e110190

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Calcium Sensing Receptor in transfected 293 cell lysate. Samples were incubated in Calcium Sensing Receptor monoclonal antibody (Product # MA1-16940). ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Calcium Sensing Receptor in rat kidney tissue. Sample was incubated in Calcium Sensing Receptor monoclonal antibody (Product # MA1-16940).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Calcium Sensing Receptor in 0.5 mg/mL Hek293 lysate. Samples were incubated in Calcium Sensing Receptor monoclonal antibody (Product # MA1-16940). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Serotonin deficiency alters the protein abundance and localization of basolateral (CaSR and ORAI1) and apical (PMCA2) calcium transporters in the mammary epithelial cells on d10 of lactation. Wild-type (WT) mouse dams were given saline and Tryptophan hydroxylase knock-out dams given either saline ( Tph1 -/- ) or daily injections of exogenous 5-HTP (100 mg/kg; Tph1 -/- (5-HTP)), from day 15 of gestation to day 10 of lactation. Representative mammary gland slides (n = 3) immuno stained for calcium sensing receptor (CaSR, red) co-satined with mucin 1 (MUC1, green, apical membrane marker, A1-6), calcium release-activated calcium channel protein 1 (ORAI-1, red) co-satined with MUC1 (B1-6), and plasma membrane Calcium ATPase and 2 (PMCA2, red) co-stained with beta-Catenin (beta-Cat, C1-6) on d10 of lactation. Blue staining = DAPI (nuclear stain) Green staining = MUC1 or beta-Cat. White arrows indicate the localization of the receptors in the alveoli.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Calcium-sensing receptor (CaSR) protein expression in the cochlea. Representative western blot of cochlea (left) and kidney (right) lysates from 5-week-old Wistar/ST rats with an anti-CaSR antibody. ss-actin was used as a loading control (lower lanes).