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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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- Product number
- GTX101127 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101127, RRID:AB_10729290
- Product name
- Lamin A + C antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
Panobinostat sensitizes KRAS-mutant non-small-cell lung cancer to gefitinib by targeting TAZ.
Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response.
Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.
A low-salt diet increases the expression of renal sirtuin 1 through activation of the ghrelin receptor in rats.
Urinary miR-16 transactivated by C/EBPβ reduces kidney function after ischemia/reperfusion-induced injury.
Alterations in nuclear structure promote lupus autoimmunity in a mouse model.
Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
Extracellularly Extruded Syntaxin-4 Is a Potent Cornification Regulator of Epidermal Keratinocytes.
Epidermal growth factor stimulates nuclear factor-κB activation and heme oxygenase-1 expression via c-Src, NADPH oxidase, PI3K, and Akt in human colon cancer cells.
Krüppel-like factor 5 mediates proinflammatory cytokine expression in lipopolysaccharide-induced acute lung injury through upregulation of nuclear factor-κB phosphorylation in vitro and in vivo.
CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.
Activating transcription factor 3 protects against pressure-overload heart failure via the autophagy molecule Beclin-1 pathway.
The A2A adenosine receptor is a dual coding gene: a novel mechanism of gene usage and signal transduction.
Productive replication of human papillomavirus 31 requires DNA repair factor Nbs1.
FJU-C4, a new 2-pyridone compound, attenuates lipopolysaccharide-induced systemic inflammation via p38MAPK and NF-κB in mice.
Identification of the proteins required for fatty acid desaturation in zebrafish (Danio rerio).
Adiponectin ameliorates iron-overload cardiomyopathy through the PPARα-PGC-1-dependent signaling pathway.
Lipocalin 2, a new GADD153 target gene, as an apoptosis inducer of endoplasmic reticulum stress in lung cancer cells.
Yu HI, Chou HC, Su YC, Lin LH, Lu CH, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Lee YR, Chan HL
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350
Panobinostat sensitizes KRAS-mutant non-small-cell lung cancer to gefitinib by targeting TAZ.
Lee WY, Chen PC, Wu WS, Wu HC, Lan CH, Huang YH, Cheng CH, Chen KC, Lin CW
International journal of cancer 2017 Nov 1;141(9):1921-1931
International journal of cancer 2017 Nov 1;141(9):1921-1931
Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
Ling HH, Kuo CC, Lin BX, Huang YH, Lin CW
Experimental cell research 2017 Jan 1;350(1):218-225
Experimental cell research 2017 Jan 1;350(1):218-225
Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response.
Hung YL, Fang SH, Wang SC, Cheng WC, Liu PL, Su CC, Chen CS, Huang MY, Hua KF, Shen KH, Wang YT, Suzuki K, Li CY
Scientific reports 2017 Apr 11;7:46299
Scientific reports 2017 Apr 11;7:46299
Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.
Kuo HF, Liu PL, Chong IW, Liu YP, Chen YH, Ku PM, Li CY, Chen HH, Chiang HC, Wang CL, Chen HJ, Chen YC, Hsieh CC
PloS one 2016;11(5):e0156059
PloS one 2016;11(5):e0156059
A low-salt diet increases the expression of renal sirtuin 1 through activation of the ghrelin receptor in rats.
Yang SY, Lin SL, Chen YM, Wu VC, Yang WS, Wu KD
Scientific reports 2016 Sep 7;6:32787
Scientific reports 2016 Sep 7;6:32787
Urinary miR-16 transactivated by C/EBPβ reduces kidney function after ischemia/reperfusion-induced injury.
Chen HH, Lan YF, Li HF, Cheng CF, Lai PF, Li WH, Lin H
Scientific reports 2016 Jun 14;6:27945
Scientific reports 2016 Jun 14;6:27945
Alterations in nuclear structure promote lupus autoimmunity in a mouse model.
Singh N, Johnstone DB, Martin KA, Tempera I, Kaplan MJ, Denny MF
Disease models & mechanisms 2016 Aug 1;9(8):885-97
Disease models & mechanisms 2016 Aug 1;9(8):885-97
Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
Wu PS, Yen JH, Kou MC, Wu MJ
PloS one 2015;10(6):e0130599
PloS one 2015;10(6):e0130599
Extracellularly Extruded Syntaxin-4 Is a Potent Cornification Regulator of Epidermal Keratinocytes.
Kadono N, Hagiwara N, Tagawa T, Maekubo K, Hirai Y
Molecular medicine (Cambridge, Mass.) 2015 Jan 14;21:77-86
Molecular medicine (Cambridge, Mass.) 2015 Jan 14;21:77-86
Epidermal growth factor stimulates nuclear factor-κB activation and heme oxygenase-1 expression via c-Src, NADPH oxidase, PI3K, and Akt in human colon cancer cells.
Lien GS, Wu MS, Bien MY, Chen CH, Lin CH, Chen BC
PloS one 2014;9(8):e104891
PloS one 2014;9(8):e104891
Krüppel-like factor 5 mediates proinflammatory cytokine expression in lipopolysaccharide-induced acute lung injury through upregulation of nuclear factor-κB phosphorylation in vitro and in vivo.
Chen HL, Chong IW, Lee YC, Tsai JR, Yuan SS, Wang HM, Liu WL, Liu PL
Mediators of inflammation 2014;2014:281984
Mediators of inflammation 2014;2014:281984
CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.
Galloway JN, Shaw C, Yu P, Parghi D, Poidevin M, Jin P, Nelson DL
Human molecular genetics 2014 Nov 15;23(22):5906-15
Human molecular genetics 2014 Nov 15;23(22):5906-15
Activating transcription factor 3 protects against pressure-overload heart failure via the autophagy molecule Beclin-1 pathway.
Lin H, Li HF, Chen HH, Lai PF, Juan SH, Chen JJ, Cheng CF
Molecular pharmacology 2014 May;85(5):682-91
Molecular pharmacology 2014 May;85(5):682-91
The A2A adenosine receptor is a dual coding gene: a novel mechanism of gene usage and signal transduction.
Lee CF, Lai HL, Lee YC, Chien CL, Chern Y
The Journal of biological chemistry 2014 Jan 17;289(3):1257-70
The Journal of biological chemistry 2014 Jan 17;289(3):1257-70
Productive replication of human papillomavirus 31 requires DNA repair factor Nbs1.
Anacker DC, Gautam D, Gillespie KA, Chappell WH, Moody CA
Journal of virology 2014 Aug;88(15):8528-44
Journal of virology 2014 Aug;88(15):8528-44
FJU-C4, a new 2-pyridone compound, attenuates lipopolysaccharide-induced systemic inflammation via p38MAPK and NF-κB in mice.
Liu JS, Jung F, Yang SH, Chou SS, Huang JL, Lu CL, Huang GL, Yang PC, Lin JC, Jow GM
PloS one 2013;8(12):e82877
PloS one 2013;8(12):e82877
Identification of the proteins required for fatty acid desaturation in zebrafish (Danio rerio).
Chen YS, Luo WI, Lee TL, Yu SS, Chang CY
Biochemical and biophysical research communications 2013 Nov 1;440(4):671-6
Biochemical and biophysical research communications 2013 Nov 1;440(4):671-6
Adiponectin ameliorates iron-overload cardiomyopathy through the PPARα-PGC-1-dependent signaling pathway.
Lin H, Lian WS, Chen HH, Lai PF, Cheng CF
Molecular pharmacology 2013 Aug;84(2):275-85
Molecular pharmacology 2013 Aug;84(2):275-85
Lipocalin 2, a new GADD153 target gene, as an apoptosis inducer of endoplasmic reticulum stress in lung cancer cells.
Hsin IL, Hsiao YC, Wu MF, Jan MS, Tang SC, Lin YW, Hsu CP, Ko JL
Toxicology and applied pharmacology 2012 Sep 15;263(3):330-7
Toxicology and applied pharmacology 2012 Sep 15;263(3):330-7
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX101127 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX101127 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C(GTX101127) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.IP samples: HeLa whole cell extractA. 50 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Lamin A + C protein by 4 £gg Lamin A + C antibody (GTX101127)7.5 % SDS-PAGEThe immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (GTX101127) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Lamin A + C antibody (GTX101127) diluted at 1:250.
