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LearnALX-804-662-R200
antibody from Enzo Life Sciences
Targeting: LMNA
CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [4]
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- Product number
- ALX-804-662-R200 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-662-R200, RRID:AB_2052319
- Product name
- Progerin (human), mAb (13A4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Liquid. Neat cell culture supernatant containing 0.02% sodium azide.
- Isotype
- IgG
- Antibody clone number
- 13A4
- Vial size
- 200 µl
Submitted references Lamin A precursor induces barrier-to-autointegration factor nuclear localization.
Ageing-related chromatin defects through loss of the NURD complex.
Capanni C, Cenni V, Haraguchi T, Squarzoni S, Schüchner S, Ogris E, Novelli G, Maraldi N, Lattanzi G
Cell cycle (Georgetown, Tex.) 2010 Jul 1;9(13):2600-10
Cell cycle (Georgetown, Tex.) 2010 Jul 1;9(13):2600-10
Ageing-related chromatin defects through loss of the NURD complex.
Pegoraro G, Kubben N, Wickert U, Göhler H, Hoffmann K, Misteli T
Nature cell biology 2009 Oct;11(10):1261-7
Nature cell biology 2009 Oct;11(10):1261-7
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis (10% PAGE) of whole cell lysate of HeLa cells ectopically expressing FLAG-tagged human progerin. The membrane was cut in strips and each strip was incubated separately with the following antibodies: Lane 1: anti-Lamin A/C antibody (N18) (1:2000), Lane 2: anti-FLAG-tag antibody (M2) (1:5000), Lane 3: MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662) (1:5)
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis (7.5% PAGE) of whole cell lysate of T98G cells treated with FTI-277, a farnesyl transferase inhibitor, which causes accumulation of unprocessed lamin A (prelamin A). The membrane was cut in strips and each strip was incubated separately with the following antibodies: Lane 1: anti-lamin A/C antibody (N-18) (1:2000), Lane 2: anti-lamin A antibody (C-20) specific for prelamin A (1:500), Lane 3: MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662) (1:5)
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunoprecipitation of progerin from lysates of Hutchinson-Gilford Progeria patient and control cells using 20µl of MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662). The immunoprecipitates were separated by SDS-PAGE, blotted and incubated with MAb to Progerin (human) (13A4) (1:200). The asteriks labels the IgG band. Figure courtesy of Giovanna Lattanzi
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunocytochemistry of skin fibroblastsÊusing MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662) and DAPI. Only Hutchinson-Gilford Progeria Syndrome (HGPS) skin fibroblasts show staining.Figure courtesy of Giovanna Lattanzi
