Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-15906 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibrinogen gamma Monoclonal Antibody (4H9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15906 targets FGG in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15906 immunogen is purified recombinant fragment of human FGG expressed in E. Coli. . MA5-15906 detects FGG which has a predicted molecular weight of approximately 52kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H9
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential.
do Amaral RJFC, Zayed NMA, Pascu EI, Cavanagh B, Hobbs C, Santarella F, Simpson CR, Murphy CM, Sridharan R, González-Vázquez A, O'Sullivan B, O'Brien FJ, Kearney CJ
Frontiers in bioengineering and biotechnology 2019;7:371
Frontiers in bioengineering and biotechnology 2019;7:371
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGG using a FGG monoclonal antibody (Product # MA5-15906) against a human FGG (AA: 210-437) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGG using a FGG monoclonal antibody (Product # MA5-15906) against a human FGG (AA: 210-437) recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using FGG monoclonal antibody (Product # MA5-15906) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cerebellum tissues using FGG monoclonal antibody (Product # MA5-15906) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FGG monoclonal antibody (Product # MA5-15906) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using FGG monoclonal antibody (Product # MA5-15906) (blue) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using FGG monoclonal antibody (Product # MA5-15906) (blue) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Platelet-rich plasma (PRP) was successfully incorporated into collagen-GAG scaffolds. Collagen-GAG scaffolds (A,D,G,J) , PRP gel (B,E,H,K) and the composite scaffold (C,F,I,L) were visualized by naked eye (A-C) , histology (D-F) , immunofluorescence (G-I) , and scanning electron microscopy (J-L) . PRP-derived fibrin was evenly incorporated within the pores of collagen-GAG scaffolds. Aniline blue (blue) evidencing collagen (D,F) and Biebrich Scarlet-Acid Fucshin (red) evidencing fibrin (E,F) . Collagen-GAG strut autofluoresced in blue (G,I) and fibrinogen immunostating in green (H,I) . Star indicates the collagen-GAG strut in (J,L) , and arrows indicates the fibrin network in (K,L) .
