Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00002629-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002629-M01, RRID:AB_539165
- Product name
- Mouse Monoclonal Glucosylceramidase/GBA Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GBA - glucosidase, beta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Reduced glucocerebrosidase activity in monocytes from patients with Parkinson's disease.
Reduced glucocerebrosidase is associated with increased α-synuclein in sporadic Parkinson's disease.
Parkin-mediated ubiquitination of mutant glucocerebrosidase leads to competition with its substrates PARIS and ARTS.
Gaucher disease paradigm: from ERAD to comorbidity.
Pharmacological chaperones facilitate the post-ER transport of recombinant N370S mutant β-glucocerebrosidase in plant cells: evidence that N370S is a folding mutant.
Characterization of the ERAD process of the L444P mutant glucocerebrosidase variant.
Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
Atashrazm F, Hammond D, Perera G, Dobson-Stone C, Mueller N, Pickford R, Kim WS, Kwok JB, Lewis SJG, Halliday GM, Dzamko N
Scientific reports 2018 Oct 18;8(1):15446
Scientific reports 2018 Oct 18;8(1):15446
Reduced glucocerebrosidase is associated with increased α-synuclein in sporadic Parkinson's disease.
Murphy KE, Gysbers AM, Abbott SK, Tayebi N, Kim WS, Sidransky E, Cooper A, Garner B, Halliday GM
Brain : a journal of neurology 2014 Mar;137(Pt 3):834-48
Brain : a journal of neurology 2014 Mar;137(Pt 3):834-48
Parkin-mediated ubiquitination of mutant glucocerebrosidase leads to competition with its substrates PARIS and ARTS.
Bendikov-Bar I, Rapaport D, Larisch S, Horowitz M
Orphanet journal of rare diseases 2014 Jun 16;9:86
Orphanet journal of rare diseases 2014 Jun 16;9:86
Gaucher disease paradigm: from ERAD to comorbidity.
Bendikov-Bar I, Horowitz M
Human mutation 2012 Oct;33(10):1398-407
Human mutation 2012 Oct;33(10):1398-407
Pharmacological chaperones facilitate the post-ER transport of recombinant N370S mutant β-glucocerebrosidase in plant cells: evidence that N370S is a folding mutant.
Babajani G, Tropak MB, Mahuran DJ, Kermode AR
Molecular genetics and metabolism 2012 Jul;106(3):323-9
Molecular genetics and metabolism 2012 Jul;106(3):323-9
Characterization of the ERAD process of the L444P mutant glucocerebrosidase variant.
Bendikov-Bar I, Ron I, Filocamo M, Horowitz M
Blood cells, molecules & diseases 2011 Jan 15;46(1):4-10
Blood cells, molecules & diseases 2011 Jan 15;46(1):4-10
Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
Campeau PM, Rafei M, Boivin MN, Sun Y, Grabowski GA, Galipeau J
Blood 2009 Oct 8;114(15):3181-90
Blood 2009 Oct 8;114(15):3181-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - GBA monoclonal antibody (M01), clone 2E2 Analysis of GBA expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Glucosylceramidase/GBA Antibody (2E2.) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.