F54518

antibody from NSJ Bioreagents

Targeting: GBA GBA1, GLUC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54518

Provider

NSJ Bioreagents

Product name

Glucosylceramidase beta Antibody / GBA / GC

Antibody type

Polyclonal

Description

This highly specific Glucosylceramidase beta antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Glucosylceramidase beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) 293T, 2) HeLa, 3) LNCaP, 4) MCF7 and 5) U-2 OS cell lysate with Glucosylceramidase beta antibody. Predicted molecular weight ~60 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse lung lysate with Glucosylceramidase beta antibody. Predicted molecular weight ~60 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skeletal muscle tissue with Glucosylceramidase beta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with Glucosylceramidase beta antibody; Blue=isotype control, Green= Glucosylceramidase beta antibody.