Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22326 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GBA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2. Predicted reactivity: Mouse (84%), Rat (85%), Pig (90%), Chimpanzee (100%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.78 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of beta-glucosidase using 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a beta-glucosidase polyclonal antibody (Product # PA5-22326) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GBA was performed by separating 30 µg of MCF-7 whole cell and membrane extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GBA Polyclonal Antibody (Product # PA5-22326) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of GBA was achieved by transfecting NIH:OVCAR-3 with GBA specific siRNAs (Silencer® select Product # S501316, S534768). Western blot analysis (Fig. a) was performed using Whole cell extracts from the GBA knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GBA Polyclonal Antibody (Product # PA5-22326, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GBA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GBA Polyclonal Antibody (Product # PA5-22326) and a 60 kDa band corresponding to GBA was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), HEK-293 (Lane 4), U-87 MG (Lane 5), NIH:OVCAR-3 (Lane 6) and LNCaP (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).An uncharacterized band of ~40 kDa was also observed in Hep G2, HEK-293 and LNCaP.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GBA Polyclonal Antibody detects GBA protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. GBA stained by GBA Polyclonal Antibody (Product # PA5-22326) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GBA Polyclonal Antibody detects GBA protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. GBA stained by GBA Polyclonal Antibody (Product # PA5-22326) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using GBA (Product # PA5-22326) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.